What To-be-discard Biomaterials From ART Cycles Can Tell us About the Treatment Cycle Outcome?
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 入组人数
- 50
- 试验地点
- 1
- 主要终点
- Proteomic profile
研究概览
简要总结
Various to-be-discard material from IVF treatment cycle will be collect and examined, aiming to identify potential reasons of successful outcome or cycle failure.
详细描述
Usually around 2/3 of IVF material will be discarded at the end of each treatment cycle, including immature oocytes, abnormal fertilized zygotes, poor grading embryos, spent culture medium, etc. Next generation sequencing will be performed for DNA / RNA analysis including the length and the quantity, RNA-seq will be applied for transcriptome profiling, and protein profile will be investigated in a pool sample. By applying various diagnostic tools against those to-be-discard biomaterials, we aim to identify potential underlying reasons of indicating either for an IVF failure or a successful cycle, and try to develop a non-invasive testing method for embryo quality check; better detection of the embryo implantation potential and eventually further increase the IVF clinical outcome.
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients attending the Assisted Reproductive Technology (ART) Unit of the Chinese University of Hong Kong for ART treatment.
- •Participant able to give voluntary, written, informed consent to participate in the study.
排除标准
- •Patient using donor gamete in IVF treatment
- •Patient refuses to join and failed to giving consent for any reasons
结局指标
主要结局
Proteomic profile
时间窗: Through study completion, around 3 year
Protein profile will be investigated in a pool sample, treated samples will go for mass spectrometry (MS) data acquisition and finally destined for data analysis and interpretation.
DNA/RNA sequencing data
时间窗: Through study completion, around 3 year
DNA / RNA analysis including the length and the quantity, RNA-seq will be applied for transcriptome profiling. Next generation sequencing will be performed to determine the sequence of DNA/RNA for our collected samples.
次要结局
- Live birth rate(Around 9 months after embryo transfer)
- Clinical pregnancy rate(Around 5 weeks after embryo transfer)
- Blastocyst formation rate(5 days after fertilization)
研究者
Yiu Leung David Chan
Assited Professor
Chinese University of Hong Kong