The Influence of Gonadal Hormone Suppression on Adipocyte Lineage and the Microbiome
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Adipose Tissue
- Sponsor
- University of Colorado, Denver
- Enrollment
- 5
- Locations
- 1
- Primary Endpoint
- Accumulation of Myeloid Cells
- Status
- Terminated
- Last Updated
- 4 years ago
Overview
Brief Summary
This research study plans to learn more about the role of female sex hormones on adipose tissue (or fat) and the gut microbiome (or the organisms that are in your digestive tract). The rationale for this study is that the rate at which women gain fat (especially in the stomach region) increases after menopause. It is thought to be due to the loss of estrogen because post-menopausal women who take estrogen gain less weight than those who do not take estrogen. Gut bacteria process estrogen and help determine the types of estrogen that circulate in the body. These bacteria can be changed with lifestyle factors such as diet, and may therefore, also affect the risk of diseases that are more common in women after menopause i.e., cardiovascular disease and cancer. In this study the investigators will obtain fat biopsies before and after 6 months of ovarian hormone suppression to measure how the fat cells change with the loss of female sex hormones (e.g., medical menopause). The investigators will also obtain stool and urine samples before and every month during the study to measure changes in the microbiome.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Enrollment into the parent protocol (COMIRB 16-1479 - The impact of estrogen status on the biological function of brown adipose tissue in women measured using quantitative PET/CT; PI - Edward Melanson, PhD)
- •Participation in GnRHag intervention
Exclusion Criteria
- •Known allergy to lidocaine or other numbing medication
- •Uncontrolled bleeding disorder or inability to withhold aspirin/NSAIDs or other blood thinning medications for 7 days prior to biopsy procedure.
Outcomes
Primary Outcomes
Accumulation of Myeloid Cells
Time Frame: 8 months after enrollment of last participant
Quantification of accumulation of myeloid cells in abdominal subcutaneous adipose tissue of women before and after gonadal hormone suppression as assessed by CD45+/CD14+ staining via flow cytometry of isolated adipose stroma.
Progenitor proliferation and adipogenic potential
Time Frame: 1 year after completion of last participant
Progenitor proliferation measured by BrdU and adipogenic potential as measured by Oil Red-O staining with quantification by absorbance. Adipocyte specific gene/protein expression will be measured to confirm adipocyte status.
Gut microbial diversity
Time Frame: 1 year after completion of last participant
Comparison of gut microbial diversity before and after gonadal hormone suppression at monthly intervals as assessed by sequencing of the V4 region of 16s rRNA
Secondary Outcomes
- In Vitro Metabolic Phenotyping(2 years after completion of last participant)
- β-glucuronidase activity(2 years after completion of last participant)
- Estrogen parent: metabolite ratios(2 years after completion of last participant)