Culture System for Isolating Circulating Tumor Cells

• Conditions: Early Detection of Cancer

• Registration Number: NCT03843450
• Lead Sponsor: National Taiwan University Hospital

Brief Summary

This culture system utilizes the special affinity difference of biomedical material coating for different cells to achieve the effect of isolating cancer cells from the blood sample. The coating of the system has the characteristic that to make the WBCs adhesion, but the cancer cells in the blood sample suspend in the culture medium, which achieves the effect of separating cancer cells from the blood. The supernatant with the cancer cells can further be isolated from the cultural system for related analysis and detection to achieve early diagnosis and screening.

Detailed Description

Not available

Study Timeline

• Study Start: 2019-01-29
• Study First Submitted: 2019-02-14
• Study First Submitted QC: 2019-02-14
• Study First Posted: 2019-02-18
• Status Verified: 2020-09
• Last Update Submitted: 2020-11-04
• Last Update Posted: 2020-11-05
• Primary Completion: 2022-07-01
• Study Completion: 2022-09-26

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 90

Inclusion Criteria

1. Polycythemia patients are between the ages of 20 and 70.
2. Willing to sign the consent form under the informed consent.

Inclusion Criteria:

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Exclusion Criteria

1. Vulnerable Subjects included minors, prisoners, aborigines, pregnant women, people with mental disorders, students, and subordinates, etc.

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Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
WBCs adhesion ratio	1 hour	The whole blood will be diluted and put into the system. And the supernatant will be collected to count the number of blood cell number by the automatic blood cell count.
Cancer Cell Isolation Efficiency of The Isolation System	1 day	The percentage isolation of fluorescently-labeled A549 cell lines will be spiked into the whole blood, and the put into the system. The supernatant of the system will be collected to re-culture on the culture dishes and analyzed by fluorescence microscope.

Trial Locations

Locations (1)

National Taiwan University Hospital; 🇨🇳 Taipei, Taiwan