Generation of Haploid Stem Cells From Human Germ Cells

• Conditions: Induced Pluripotent Stem Cells
• Interventions: Other: generation of stem cells

• Registration Number: NCT01454765
• Lead Sponsor: Rambam Health Care Campus

Brief Summary

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.

Detailed Description

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.

Study Timeline

• Study First Submitted: 2011-10-16
• Study First Submitted QC: 2011-10-18
• Study First Posted: 2011-10-19
• Study Start: 2011-12
• Status Verified: 2013-12
• Last Update Submitted: 2013-12-10
• Last Update Posted: 2013-12-11
• Primary Completion: 2014-12
• Study Completion: 2015-08

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Male
• Target Recruitment: 10

Inclusion Criteria

1. males over the age of 18 and under 50
2. no known chronic illness
3. not taking any chronic drugs

Exclusion Criteria

1. females
2. males under 18 or over 50
3. any known chronic illness
4. taking chronic medications

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Sperm sample from healthy volunteers	generation of stem cells	-

Primary Outcome Measures

Name	Time	Method
Patients donated samples	June 2011-November 2013	No samples collected to date

Secondary Outcome Measures

Name	Time	Method
No secomdary outcome	June 2011 - November 2013

Trial Locations

Locations (2)

Weizmann institute of science; 🇮🇱 Rehovot, Israel

Rambam medical center; 🇮🇱 Haifa, Israel