Influence of Cupping Therapy on Immune System in Post Covid- 19 Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 76
- 试验地点
- 1
- 主要终点
- T-lymphocyte
研究概览
简要总结
Purpose of this study is to investigate
- The effect of Dry cupping therapy on T-Lymphocyte in post covid-19 patients.
- The effect of Dry cupping therapy on serum Cytokine in post covid-19 patients.
- The effect of Dry cupping therapy on Immunoglobulin IgA.
- The effect of Dry cupping therapy on Immunoglobulin IgM.
- The effect of Dry cupping therapy on Immunoglobulin IgG. seventy six patients of both genders with deficiency in T-Lymphocyte number,,higher level of serum cytokine and lower immunoglobulin IgA,IgM,IgG after two weeks of recovery from covid-19 . Age range from Twenty one to Sixty six years old .They participated in the study and recruited from surveillance unit of Shobra general hospital .These patients recruited by phone and have been offered to participate in the programme.The patients were divided equally into two groups (A and B). Assessment was done before and after treatment. T-Lymphocyte, serum cytokine and immunoglobulin IgA, IgM and IgG were analyzed by kenza 240 TX from fluorescence-labeled flow cytometry. Disposable cupping therapy instrument was used in treatment of group (A) in addition to traditional medical treatment in the form of vitamin C, D and anticoagulant drugs. Traditional medical treatment only was used in treatment of group (B).The obtained results of this study will determine the significant improvement of participated two groups.
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详细描述
Patient were selected randomly and distributed in two groups by computer generation. The sample size was calculated using the G*Power software (version 3.0.10). F-test MANOVA within and between interaction effects was selected. Considering a power of 0.80, an α level of 0.05 (2 tailed) and effect size of 0.39; two groups and response variables of five, a generated sample size of at least 38 participants per group was required and total sample size of 76 subjects.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- Single (Outcomes Assessor)
盲法说明
single
入排标准
- 年龄范围
- 21 Years 至 66 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •All patients with body mass index(BMI) 25.0-29.9 kg/m2 recruited two weeks post recovary from covid-19 and have lower T-lymphocyte and elevated cytokine All patients were clinically and medically stable.All patients recruited by phone and have been offered to participate in the programme.All patients will sign the informed consent before joining the study. Age range from 21 to 66 years .Subjects will be divided equally into two groups A,B. Assessment will be done before and after treatment.
排除标准
- •Patients will be excluded if they have any of the following criteria:
- •History of acute or chronic infections
- •Hepatobiliary diseases
- •Hematological diseases
- •Urinary system diseases
- •Nutrition and metabolism diseases
- •Rheumatic diseases
- •Endocrine diseases
- •Circulatory system diseases
- •Muscle trauma
- •Hypertension Further, if they fulfilled any of the following testing criteria
- •Hepatitis C virus antibodies
- •Human immunodeficiency virus antibodies
- •Creatinine above 120 μmol/L
- •Creatine kinase above 500 U/L
- •Uric acid above 475 μmol/L, glucose above 7.0 mmol/L
- •C-reactive protein above 12.0 m.
研究组 & 干预措施
Cupping therapy with Convential medical treatment
Cupping therapy with Convential medical treatment
干预措施: Cupping therapy with convential medical treatment (Combination Product)
Cupping therapy with Convential medical treatment
Cupping therapy with Convential medical treatment
干预措施: Convential medical treatment (Drug)
Convential medical treatment
Convential medical treatment
干预措施: Cupping therapy with convential medical treatment (Combination Product)
Convential medical treatment
Convential medical treatment
干预措施: Convential medical treatment (Drug)
结局指标
主要结局
T-lymphocyte
时间窗: 3months
1- Lymphocyte subset were analyzed from fluorescence-labeled flow cytometry on a DxFLEX flow cytometry by Kenza 240 Tx
Serum Cytokine
时间窗: 3months
Cytokine detection Kenza reagents were provided from a 240 TX bio lap diagnostic apparatus by microsphere flow immunofluorescence
ImmunoglobulinA,M,G
时间窗: 3 months
Kenza 240 TX for detection of immunoglobulin
次要结局
未报告次要终点
研究者
Marwa Mohamed Abd ElZahr
Dr
Cairo University