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The Effect of a Probiotic Strain on Aspirin-induced GI Damage.

Phase 2
Completed
Conditions
Side Effects of Acetylsalicylic Acid Use
Interventions
Dietary Supplement: Placebo
Dietary Supplement: probiotic strain
Registration Number
NCT03228589
Lead Sponsor
Chr Hansen
Brief Summary

This trial is a single-site, randomised, double-blind, placebo-controlled, two-armed, parallel-group trial in healthy, adult volunteers. The trial will investigate the effect of daily intake of a probiotic strain versus placebo when co-administered to daily intake of 300mg of Aspirin. The objective is to investigate the ability of the probiotic strain to attenuate and/or reverse Aspirin-induced deterioration of the small intestine.

Detailed Description

The trial includes a run-in period of two weeks duration followed by a six weeks intervention period where a probiotic strain/placebo and Aspirin is co-administered. After the 6 weeks, probiotic strain/placebo is given for two additional weeks to investigate the potential effects of the probiotic strain on intestinal healing after long-time Aspirin use.

Subjects will participate in the trial for a total duration of 10 weeks including the run-in phase. Besides the screening visit, the trial will consist of 6 visits.

After having given their written informed consent, subjects will complete screening procedures to evaluate their eligibility for participation in the trial and complete a run-in period of two weeks duration to washout possible pre-trial probiotics and/or use of medication. After baseline assessments at Visit 2, subjects will start daily intake of 300mg Aspirin and also be randomly assigned to 8 weeks daily intake of active or placebo product in a ratio of 1:1.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
109
Inclusion Criteria
  • Written informed consent
  • Healthy and without any gastrointestinal discomfort/pain symptoms
  • Age ≥ 18 - ≤ 40 years of both gender (aim of 1/3 of each gender in each arm)
  • Sedentary lifestyle (weekly training load below 2 hours within endurance sports)
  • Willing to abstain from any other probiotic products and/or medication known to alter gastrointestinal function throughout the participation of the trial
Exclusion Criteria
  • Abdominal surgery which, as judged by the investigator, might affect the GI function (except appendectomy and cholecystectomy)
  • History of peptic ulcer disease
  • Any known bleeding disorder
  • Allergy to Aspirin
  • History of H. pylori disease
  • Resting diastolic blood pressure ≥ 90 mmHg
  • Resting systolic blood pressure ≥ 140 mmHg
  • A current diagnosis of psychiatric disease
  • Systemic use of antibiotics, steroids (except contraceptives) or antimicrobial medication in the last 2 months
  • BMI > 27
  • Daily usage of non-steroidal anti-inflammatory drugs in the last 2 months or incidental use in the last 2 weeks prior to screening (Aspirin, Ibuprofen, Diclofenac, Naproxen, Celecoxib, Mefenamic acid, Etoricoxib, Indometacin)
  • Usage of medications, except contraceptives, in the last 2 weeks prior to screening
  • Diagnosed inflammatory gastrointestinal disease and/or irritable bowel syndrome
  • Lactose intolerance
  • Any other disease that, by the Investigators discretion, could interfere with the intestinal barrier function of the subject
  • Participation in other clinical trials in the past 2 months prior to screening
  • Regular use of probiotics in the last 2 months
  • Smoking and/or frequent use of other nicotine products
  • Desire and/or plans on changing current diet and/or exercise regime during the participation of this trial
  • Use of laxatives, anti-diarrheals, anti-cholinergics and PPI within last 2 months prior to screening
  • Use of immunosuppressant drugs within last 4 weeks prior to screening
  • For Women: Pregnancy or lactation

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboPlacebo arm treated with placebo capsules (identical to active product capsule besides the bacteria) for 8 weeks.
probiotic strainprobiotic strainActive arm treated with the probiotic strain for 8 weeks.
Primary Outcome Measures
NameTimeMethod
Lewis score from capsule endoscopy8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of the capsule endoscopy Lewis score between Visit 2 (randomization) and Visit 7 (end of treatment).

Secondary Outcome Measures
NameTimeMethod
Mucosal ulcer number8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of the capsule endoscopy mucosal total ulcer number between Visit 2 (randomization) and Visit 7 (end of treatment).

Pain module score of the GSRS questionnaire8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on gastrointestinal symptoms when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of pain module score of the GSRS questionnaire between Visit 2 (randomization) and Visit 7 (end of treatment).

Blood I-FABP concentration8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve for Visit 2-Visit 7 blood I-FABP concentration.

Total score of the GSRS questionnaire8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on gastrointestinal symptoms when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of total score of the GSRS questionnaire between Visit 2 (randomization) and Visit 7 (end of treatment).

Mucosal red spots number8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of the capsule endoscopy mucosal red spot number between Visit 2 (randomization) and Visit 7 (end of treatment).

Feces Calprotectin concentration.8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve for Visit 2-Visit 7 feces Calprotectin concentration.

blood Calprotectin concentration8 weeks

The effect of 8 weeks oral supplementation of probiotic strain versus placebo on small intestinal mucosa damage when co-administered to a 6 week Aspirin challenge measured as the area-under-the-curve of the blood Calprotectin concentration between Visit 2 (randomization) and Visit 7 (end of treatment).

Trial Locations

Locations (1)

Atlantia Food Clnical Trials

🇮🇪

Cork, Ireland

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