Proteomic Study of Plasma Exosomes in Patients With Diabetic Retinopathy
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Diabetic Retinopathy
- Sponsor
- Xuanwu Hospital, Beijing
- Enrollment
- 24
- Primary Endpoint
- differential proteins of exosomes
- Status
- Not yet recruiting
- Last Updated
- 2 years ago
Overview
Brief Summary
Objective: Explore the biomarkers related to proliferative diabetic retinopathy by analyzing the protein expression profile changes of plasma exosomes in patients with difference phases of diabetic retinopathy, and investigate potential molecular mechanisms and therapeutic targets. Methods: Enrich exosomes from plasma by column extraction method. NTA, WB and TEM were used to characterize the obtained exosomes, and exosome proteinomics was performed by chromatography⁃mass spectrometry. Corhorts: The study was divided into four groups: a healthy control group, a diabetic without diabetic retinopathy group, a non-proliferative diabetic retinopathy group, and a proliferative diabetic retinopathy group, with 6 cases in each group.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Healthy volunteers without diabetes/ Senile cataract patients without diabetes；
- •Patients with diabetes who are diagnosed without DR, NPDR, and PDR by mydriatic fundus photography (or FFA)；
Exclusion Criteria
- •Patients with systemic diseases such as cancer, immune diseases, infectious diseases, and blood diseases；
- •Patients with cerebral infarction, myocardial infarction and other cardiovascular and cerebrovascular diseases
Outcomes
Primary Outcomes
differential proteins of exosomes
Time Frame: December,2023-June,2024
Exosomes were extracted and isolated from plasma, proteomics analysis of proteins in exosomes was performed to compare the number of types of changes in exosomal proteins of the four groups and to identify proteins that were up-regulated and/or down-regulated, and the relationship between the proteins and diabetic retinopathy was then analysed to look for possible mechanisms and therapeutic targets for diabetic retinopathy.