Proteomic Study of Plasma Exosomes in Patients With Diabetic Retinopathy

Not yet recruiting

• Conditions: Diabetic Retinopathy

• Registration Number: NCT06188013
• Lead Sponsor: Xuanwu Hospital, Beijing

Brief Summary

Objective: Explore the biomarkers related to proliferative diabetic retinopathy by analyzing the protein expression profile changes of plasma exosomes in patients with difference phases of diabetic retinopathy, and investigate potential molecular mechanisms and therapeutic targets. Methods: Enrich exosomes from plasma by column extraction method. NTA, WB and TEM were used to characterize the obtained exosomes, and exosome proteinomics was performed by chromatography⁃mass spectrometry. Corhorts: The study was divided into four groups: a healthy control group, a diabetic without diabetic retinopathy group, a non-proliferative diabetic retinopathy group, and a proliferative diabetic retinopathy group, with 6 cases in each group.

Detailed Description

Not available

Study Timeline

• Status Verified: 2023-11
• Study First Submitted: 2023-12-11
• Study First Submitted QC: 2024-01-01
• Last Update Submitted: 2024-01-01
• Study Start: 2024-01-02
• Study First Posted: 2024-01-03
• Last Update Posted: 2024-01-03
• Primary Completion: 2024-03-31
• Study Completion: 2024-06-30

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 24

Inclusion Criteria

• Healthy volunteers without diabetes/ Senile cataract patients without diabetes；
• Patients with diabetes who are diagnosed without DR, NPDR, and PDR by mydriatic fundus photography (or FFA)；

Exclusion Criteria

• Patients with systemic diseases such as cancer, immune diseases, infectious diseases, and blood diseases；
• Patients with cerebral infarction, myocardial infarction and other cardiovascular and cerebrovascular diseases

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
differential proteins of exosomes	December,2023-June,2024	Exosomes were extracted and isolated from plasma, proteomics analysis of proteins in exosomes was performed to compare the number of types of changes in exosomal proteins of the four groups and to identify proteins that were up-regulated and/or down-regulated, and the relationship between the proteins and diabetic retinopathy was then analysed to look for possible mechanisms and therapeutic targets for diabetic retinopathy.