Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Protein hydrolysate amino acid label time to peak appearance in plasma
研究概览
简要总结
10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.
The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.
Hypotheses:
I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.
II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •young healthy adults,
- •organised sport/training 1-3 times per week
排除标准
- •Subject younger than 18 years or above 30 years
- •BMI > 30
- •Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
- •Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
- •Participating in systematized exercise more than 3 times per week.
- •Vegetarian.
- •Participating in a special diet which deviates from the standard of young adults.
研究组 & 干预措施
Meat hydrolysate & Cluster Dextrin
Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin.
干预措施: Meat Hydrolysate (Dietary Supplement)
Meat hydrolysate & Cluster Dextrin
Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin.
干预措施: Cluster Dextrin (Dietary Supplement)
Meat hydrolysate & placebo
Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.
干预措施: Meat Hydrolysate (Dietary Supplement)
Meat hydrolysate & placebo
Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.
干预措施: Placebo (Dietary Supplement)
结局指标
主要结局
Protein hydrolysate amino acid label time to peak appearance in plasma
时间窗: From hour 0 to hour 3
Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma
次要结局
- Insulin concentration(From hour 0 to hour 3)
- Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise(from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3.)
- Protein hydrolysate amino acid label area under the concentration curve in plasma(From hour 0 to hour 3)