Influence of Carbohydrate on Amino Acid Absorption From Dietary Protein (ICADP)
- Conditions
- Muscle Protein SynthesisAmino Acid
- Interventions
- Dietary Supplement: Meat HydrolysateDietary Supplement: Cluster DextrinDietary Supplement: Placebo
- Registration Number
- NCT03303729
- Lead Sponsor
- Bispebjerg Hospital
- Brief Summary
10 young males will be recruited to participate in a randomized double blinded crossover study to investigate the influence of concomitant intake of two different types of carbohydrate with protein hydrolysate on the absorption kinetics of protein-derived amino acids and the impact on myofibrillar protein synthesis. During the trial days the subjects will perform a bout of whole body resistance exercise and ingest a supplement of meat hydrolysate labeled with D5-phenylalanine together with randomized and double blinded type of carbohydrate consisting of either glucose or cluster dextrin.
The primary outcome is the time to obtain peak concentration of D5-phenylalanine after intake of meat hydrolysate with either glucose or cluster dextrin.
Hypotheses:
I) The cluster dextrin will increase the absorption of the amino acids from the meat hydrolysate, and thereby result in a faster rise and a higher peak in plasma amino acid concentration in the postprandial period.
II) The meat hydrolysate will increase the protein synthesis rate to a higher extent when combined with cluster dextrin than with glucose.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- Male
- Target Recruitment
- 10
- young healthy adults,
- organised sport/training 1-3 times per week
- Subject younger than 18 years or above 30 years
- BMI > 30
- Chronic medical diseases: active cancer, renal diseases, metabolic diseases like diabetes mellitus.
- Physical inactivity. Subjects must participate in systematized weekly exercise 1-3 times per week.
- Participating in systematized exercise more than 3 times per week.
- Vegetarian.
- Participating in a special diet which deviates from the standard of young adults.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Meat hydrolysate & Cluster Dextrin Cluster Dextrin Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin. Meat hydrolysate & Cluster Dextrin Meat Hydrolysate Meat hydrolysate containing 25g of protein given together with 75 grams of Cluster Dextrin. Meat hydrolysate & placebo Meat Hydrolysate Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose. Meat hydrolysate & placebo Placebo Meat hydrolysate containing 25g of protein given together with 75 grams of Glucose.
- Primary Outcome Measures
Name Time Method Protein hydrolysate amino acid label time to peak appearance in plasma From hour 0 to hour 3 Difference between the time to peak of protein-derived D5-phenylalanine amino acid appearance in the plasma
- Secondary Outcome Measures
Name Time Method Insulin concentration From hour 0 to hour 3 Difference between insulin concentrations after intake of the two carbohydrate types.
Myofibrillar protein fractional synthesis rate response to protein and carbohydrate intake after a whole body resistance exercise from hour 0,5 to hour hour 1. From hour 1 to hour 3. From hour 0,5 to hour 3. Difference between myofibrillar protein fractional synthesis rate after meat hydrolysate with either 75 grams of Cluster Dextrin or Clucose.
Protein hydrolysate amino acid label area under the concentration curve in plasma From hour 0 to hour 3 Difference between the area under the concentration curve of protein-derived D5-phenylalanine amino acid appearance in the plasma
Trial Locations
- Locations (1)
Bispebjerg Hospital
🇩🇰Copenhagen, Copenhagen NV, Denmark