Vitamin D2, Muscle Damage, NASCAR Pitcrew

Not Applicable

Completed

• Conditions: Muscle Function; Muscle Soreness; Vitamin D Status
• Interventions: Dietary Supplement: Vitamin D2 mushroom powder; Other: Placebo

• Registration Number: NCT01825616
• Lead Sponsor: Appalachian State University

Brief Summary

Hypothesis: Six weeks of supplementation with vitamin D (4000 IU/day) using Dole's Vitamin D Portobello Mushroom Powder will increase winter serum vitamin D levels, and improve muscle function and strength, and innate immunity (granulocyte/monocyte phagocytosis and oxidative burst activity), and attenuate exercise-induced muscle damage and DOMS.

Detailed Description

Vitamin D2 is found naturally in sun-exposed mushrooms, and vitamin D3 is synthesized in the skin when exposed to sunlight and is present in oil-rich fish such as salmon, mackerel, and herring. Mushrooms contain very little or any vitamin D2 but are abundant in ergosterol, which can be converted into vitamin D2 by ultraviolet (UV) illumination (Eur J Clin Nutr. 2011;65:965-71).

In recent decades, there has been increased awareness of the impact of vitamin D on muscle function (Scand J Med Sci Sports. 2010;20:182-90). In the early 20th century, athletes and coaches felt that ultraviolet rays had a positive impact on athletic performance, and evidence is accumulating to support this view. Both cross-sectional and longitudinal studies support a functional role for vitamin D in muscle, and the discovery of the vitamin D receptor in muscle tissue provides a mechanistic pathway for understanding the role of vitamin D within muscle. Studies in athletes have found that vitamin D status is variable and is dependent on outdoor training time (during peak sunlight), skin color, and geographic location (Pediatr Clin North Am. 2010;57:849-61).

25(OH)D is the best indicator and major form of vitamin D in the blood, with a circulating half-life of 2-3 weeks. Vitamin D deficiency is defined as a plasma vitamin D \[25(OH)D\] level of less than 20 ng/ml, with vitamin D insufficiency defined as 21-29 ng/ml. Estimates are that 20-100% of children, young and middle-aged adults, and community-dwelling elderly men and women are vitamin D deficient (J Clin Endocrinol Metab. 2011;96:1911-30).

Purpose: To determine if 6 weeks supplementation with Dole Vitamin D Portobello Mushroom Powder (4,000 IU/day) can, 1) increase winter serum vitamin D levels, and measure whether vitamin D supplementation 2) has a chronic influence on muscle function and strength, and innate immune function, and 3) can attenuate exercise-induced muscle damage and delayed onset of muscle soreness (DOMS) in athletes.

Study Timeline

• Study Start: 2012-10
• Primary Completion: 2013-01
• Study Completion: 2013-01
• Status Verified: 2013-04
• Study First Submitted: 2013-04-03
• Study First Submitted QC: 2013-04-03
• Study First Posted: 2013-04-05
• Last Update Submitted: 2013-04-08
• Last Update Posted: 2013-04-09

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 33

Inclusion Criteria

• NASCAR pit crew members
• Willing to avoid food and supplement sources (during the 6-week supplementation period) that are high in vitamin D (specifically canned fish, cod liver oil, salmon, and supplements with high-dose vitamin D).
• Agree to train normally.
• Avoid the use of large dose vitamin/mineral supplements (above 100% recommended dietary allowances), and medications known to affect immune function.
• Avoid the use of tanning beds.
• Willing to adhere to all aspects of the study design.

Exclusion Criteria

• Not allergic to mushrooms.
• Do not have a heart problem or have been told by your doctor not to engage in vigorous exercise.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Vitamin D2 mushroom powder	Vitamin D2 mushroom powder	4000 IU/day vitamin D2 mushroom powder
Placebo	Placebo	Mushroom powder without vitamin D2 (not exposed to UV radiation)

Primary Outcome Measures

Name	Time	Method
Muscle damage	6 weeks	myoglobin (ug/L) and creatine kinase (IU/L) after eccentric resistance exercise
Muscle Function	6 weeks	Bench press of body weight, reps to fatigue Vertical jump (watts( 300 yard shuttle run (seconds) 30-sec Wingate anaerobic power test (watts/kg)

Secondary Outcome Measures

Name	Time	Method
Serum vitamin D concentration	6 weeks	25(OH)D2 and 25(OH)D3
Immune Function	6 weeks	White blood cell differential Myeloperoxidase Immune cell cytotoxicity

Trial Locations

Locations (3)

ASU-NCRC Human Performance Laboratory; 🇺🇸 Kannapolis, North Carolina, United States

ASU Human Performance Laboratory, North Carolina Research Campus; 🇺🇸 Kannapolis, North Carolina, United States

ASU-NCRC Human Performance Lab; 🇺🇸 Kannapolis, North Carolina, United States