The effect of different radiotherapy regimen on the immune cell landscape
Recruiting
- Conditions
- cancertumor10027656
- Registration Number
- NL-OMON52433
- Lead Sponsor
- Radboud Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 200
Inclusion Criteria
- treated with radiotherapy for cancer at the Radboudumc
- understand dutch
- mentally competent
- 30-80 years old
Exclusion Criteria
- autoimmune disease
- immune- suppressive drugs (with the exception of <= 10 mg prednisone or
equivalent doses of corticosteroids).
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Immune cells will be characterized by flow cytometry, including CD4, CD8 and<br /><br>regulatory T cells, Natural Killer cells, MDSCs and macrophages. We will also<br /><br>look at the activation markers such as CD28, CD137 and CD66b, and the<br /><br>inhibitory markers PD1, PDL-1. RNA will be isolated from the immune cells for<br /><br>any specific gene expression analyzes or RNAseq. The effect of the various<br /><br>radiotherapy regimens on the dynamics of these immune cells will be determined.</p><br>
- Secondary Outcome Measures
Name Time Method <p>Above mentioned immune cell markers will be quantified in biopsies using<br /><br>immunohistochemistry. The microbiome will be examined in faeces.</p><br>