Hand Eczema in the Health Care Sector
Trial Snapshot
- Phase
- Not Applicable
- Sponsor
- Enrollment
- 300
- Locations
- 1
- Primary Endpoint
- Staphyloccous aureus presence
Study Overview
Brief Summary
This project aims to investigate the prevalence of bacterial colonization and associated factors in health care workers with hand eczema with focus on Staphylococcus aureus.
Detailed Description
Two groups will be examined in this study: health care workers with HE and matched controls invited through a questionnaire study which will be sent to approximately 4000 health care workers in Capital Region. Samples from health care workers with and without hand eczema will be collected after obtaining their written informed consent. Collection of samples: Swabs will be rubbed on the skin for 30 seconds on lesional and non-lesional skin on the dominating hand and from the nostrils. The swabs will immediately be stored in -80 C° (research biobank) for subsequent analysis at Statens Serum Institute.
Study Design
- Study Type
- Observational
- Observational Model
- Case Control
- Time Perspective
- Cross Sectional
Eligibility Criteria
- Ages
- 18 Years to 70 Years (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •The diagnosis of hand eczema (for group with hand eczema)
Exclusion Criteria
- •Other skin diseases (in both groups)
Outcomes
Primary Outcomes
Staphyloccous aureus presence
Time Frame: Baseline, cross-sectional
Prevalence of S. aureus colonization on hands and in nose in two groups of health care workers. The swab samples will be assessed by culturing on selective S. aureus plates (chromID S. aureus; bioMerieux, Marcy l'Etoile, France) and incubated overnight at 37 °C. DNA will be purified from S. aureus isolates using the Qiagen DNeasy Blood and Tissue Purification Kit (Qiagen, Hilden, Germany).
Secondary Outcomes
- CC-types and spa-typing(Baseline, cross-sectional)
- Hand Eczema Severity Index (HECSI)(Baseline, cross-sectional)
Investigators
Yasemin Topal Yüksel
MD
University Hospital Bispebjerg and Frederiksberg