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Fermented Iron-rich Supplement in Reducing Anemia

Phase 2
Conditions
Iron Deficiency Anemia
Interventions
Dietary Supplement: iron rich food supplement (60 mg iron)
Dietary Supplement: supplement containing 60 mg iron sulfate
Dietary Supplement: iron rich food supplement (10 mg iron)
Dietary Supplement: supplement containing 10 mg iron sulfate
Registration Number
NCT02037724
Lead Sponsor
University of Ghana
Brief Summary

The consequences of iron deficiency anemia in women are enormous, and especially in developing countries, as the condition adversely affects both their productive and reproductive capabilities. The study seeks to: 1) compare changes in iron status indicators among women receiving an iron-rich organic food supplement versus ferrous sulfate supplement, and 2) determine the suitable level of food supplement needed to prevent/reduce iron deficiency anemia among women in developing country settings.

A double-blind, randomized, controlled, intervention trial will be implemented in women of childbearing age, 60 women with iron deficiency anemia and 60 women with iron deficiency. After screening potential subjects (up to 500 expected), approximately 30 will be recruited into each of four study groups; assuming 30% dropout rate, to detect an increase of 30% in ferritin as significant between the two time points at 80% power and alpha value of 0.05. Subjects who meet the inclusion criteria will be randomized into the four groups consisting of: 2 control groups (daily 60mg ferrous sulfate (FS-60) or daily 10mg ferrous sulfate (FS-10)), and 2 test groups (daily 60mg iron-rich supplement (IRS-60) or 10mg iron-rich supplement (IRS-10)). Subjects will take daily FS-60 and IRS-60 under supervision for 8 weeks while subjects taking FS-10 and IRS-10 will take the supplement under supervision for 12 consecutive weeks.

Detailed Description

The design will involve screening of about 500 female adult students and staff of the University of Ghana who meet initial the inclusion criteria. Screening will involve pregnancy testing, assessment of hemoglobin, and ferritin and also administration of a health history questionnaire.

Thereafter, 120 eligible subjects will enrolled and randomized to recieve the treatment. All subjects will complete 3-day dietary record (including 2 weekdays and 1 weekend) at baseline and at endline (i.e. one week prior to treatment completion). The dietary data will be analyzed using nationally-generated food composition tables together with the ESHA food processor software. In addition, body weight and height will be measured using standard methods at baseline and end of the study. Weekly monitoring of side effects will be recorded. Fasting blood samples will be collected at baseline and endline to enable determination of anemia status and other indicators including serum ferritin, transferrin receptor and full blood count, together with C-reactive protein.

Recruitment & Eligibility

Status
UNKNOWN
Sex
Female
Target Recruitment
120
Inclusion Criteria
  • 18-49 years
  • Regular menstruation in the last three months
  • Hemoglobin <12 mg/dl;
  • Serum Ferittin<20mcg/L
  • BMI 18.5Kg/m^2 to 29.9 kg/m^2
Exclusion Criteria
  • history of gastrointestinal or hematological disorders,
  • taking medications that could interfere with hematopoiesis or dietary iron absorption
  • pregnant (based on pregnancy test).

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
supplement containing 60 mg iron sulfateiron rich food supplement (60 mg iron)nutrient supplement containing 60 mg of iron as ferous sulfate
supplement containing 10 mg iron sulfateiron rich food supplement (60 mg iron)nutrient supplement containing 10 mg of iron as ferous sulfate
iron rich food supplement (60 mg iron)supplement containing 60 mg iron sulfatecontains 60 mg Iron
iron rich food supplement (60 mg iron)iron rich food supplement (10 mg iron)contains 60 mg Iron
supplement containing 60 mg iron sulfatesupplement containing 10 mg iron sulfatenutrient supplement containing 60 mg of iron as ferous sulfate
iron rich food supplement (60 mg iron)supplement containing 10 mg iron sulfatecontains 60 mg Iron
iron rich food supplement (10 mg iron)iron rich food supplement (60 mg iron)contains 10 mg of iron
supplement containing 60 mg iron sulfateiron rich food supplement (10 mg iron)nutrient supplement containing 60 mg of iron as ferous sulfate
iron rich food supplement (10 mg iron)supplement containing 60 mg iron sulfatecontains 10 mg of iron
supplement containing 10 mg iron sulfatesupplement containing 60 mg iron sulfatenutrient supplement containing 10 mg of iron as ferous sulfate
iron rich food supplement (10 mg iron)supplement containing 10 mg iron sulfatecontains 10 mg of iron
supplement containing 10 mg iron sulfateiron rich food supplement (10 mg iron)nutrient supplement containing 10 mg of iron as ferous sulfate
Primary Outcome Measures
NameTimeMethod
iron deficiency anemiatwo and three months

Improvement of iron status with 60 mg (after two months) and 10 mg iron (after 3 months) will be assessed. The iron status measurements include, hemoglobin, ferritin, transferrin receptor and whole blood count

Secondary Outcome Measures
NameTimeMethod
comparison of side effects of iron supplement3 months

We expect to have less side effects with IRS compared with ferrous sulfate (as observed in previous studies) that include: constipation, diarrhea, vomiting, gastrointestinal distress, change in stool color and others. We will assess these with a questionnaire.

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