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A First-in-human Study Looking at the Safety of ZP8396 and How it Works in the Body of Healthy Trial Participants

Phase 1
Completed
Conditions
Healthy Volunteers
Overweight
Interventions
Drug: Placebo (ZP8396)
Registration Number
NCT05096598
Lead Sponsor
Zealand Pharma
Brief Summary

The research study will investigate the safety and tolerability of ZP8396 in healthy study participants. In addition, the study will investigate how ZP8396 works in the body (pharmacokinetics and pharmacodynamics).

Participants will receive 1 single dose either as an injection under the skin (subcutaneous, s.c.) or an injection into a vein of one arm (intravenous, i.v.).

Participants will have 9 visits with the study team. One of these visits consists of 8 overnight stays at the study site. For each participant, the study will last up to 66 days.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
64
Inclusion Criteria
  • Healthy male subject
  • Body Mass Index (BMI) between 21.0 and 29.9 kg/m^2, both inclusive
  • Body weight of at least 70.0 kg
  • Glycosylated hemoglobin A1c (HbA1c) less than 5.7 percent
  • Further inclusion criteria apply
Exclusion Criteria
  • History of metabolic diseases more frequently associated with obesity, e.g. type-2-diabetes mellitus, hypertension, dyslipidemia, heart disease or stroke
  • Systolic blood pressure < 90 mmHg or >139 mmHg and/or diastolic blood pressure less than 50 mmHg or greater than 89 mmHg
  • Symptoms of arterial hypotension
  • Further exclusion criteria apply

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
ZP8396ZP8396Up to 10 single dose cohorts are planned with 8 subjects in each; 6 participants in each cohort will receive active treatment.
Placebo (ZP8396)Placebo (ZP8396)In each of the 10 single dose cohorts, 2 subjects will receive placebo.
Primary Outcome Measures
NameTimeMethod
Incidence of treatment emergent adverse events (TEAEs)From dosing (Day 1) to end of trial (Day 50)
Secondary Outcome Measures
NameTimeMethod
Pharmacokinetics (PK) of ZP8396 (Cmax)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Maximum (peak) plasma drug concentration

Pharmacokinetics (PK) of ZP8396 (tmax)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Time to reach maximum (peak) plasma concentration

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCacetaminophen, 0-240 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the acetaminophen concentration-time curve from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacokinetics (PK) of ZP8396 (Vz/f)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Apparent volume of distribution during terminal phase

Pharmacokinetics (PK) of ZP8396 (CL)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Total body clearance of the drug (i.v. only)

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Cmax, Plasma Glucose [PG])0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Maximum PG concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Tmax, insulin)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Time to maximum insulin concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacokinetics (PK) of ZP8396 (AUClast)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Area under the plasma concentration-time curve from time zero to the time of the last measurable concentration

Pharmacokinetics (PK) of ZP8396 (AUCτ)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Area under the plasma concentration-time curve over a dosing interval

Pharmacokinetics (PK) of ZP8396 (Vz)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Volume of distribution during the terminal phase (i.v. only)

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Tmax, Plasma Glucose [PG])0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Time to maximum PG concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCglucagon,0-60 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the glucagon concentration-time curve from 0 to 60 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacokinetics (PK) of ZP8396 (MRT)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Mean residence time

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Cmax acetaminophen)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Maximum acetaminophen concentration after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCacetaminophen, 0-60 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the acetaminophen concentration-time curve from 0 to 60 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacokinetics (PK) of ZP8396 (AUCinf)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Area under the plasma concentration-time curve from time zero to infinity

Pharmacokinetics (PK) of ZP8396 (λz)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Elimination rate constant

Pharmacokinetics (PK) of ZP8396 (t½)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Elimination half-life

Pharmacokinetics (PK) of ZP8396 (CL/f)Day 1 (pre-dose) to Day 50 (1176 hours post-dose)

Apparent total clearance of the drug from plasma

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Tmax acetaminophen)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Time to maximum acetaminophen concentration after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCPG,0-60 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the Plasma Glucose (PG) concentration-time curve from 0 to 60 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCinsulin,0-60 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the insulin concentration-time curve from 0 to 60 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Cmax, glucagon)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Maximum glucagon concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCPG,0-240 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the Plasma Glucose (PG) concentration-time curve from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Cmax, insulin)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Maximum insulin concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCinsulin,0-240 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the insulin concentration-time curve from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (AUCglucagon,0-240 min)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Area under the glucagon concentration-time curve from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Pharmacodynamics (PD) of ZP8396, for dose cohorts greater than or equal to 0.7 mg (Tmax, glucagon)0-240 minutes, relative to ingestion of MTM/acetaminophen on Day -1 and Day 5

Time to maximum glucagon concentration from 0 to 240 minutes after ingestion of a standardised Mixed Test Meal (MTM) and 1000 mg acetaminophen

Trial Locations

Locations (1)

Profil Institut für Stoffwechselforschung GmbH

🇩🇪

Neuss, North Rhine-Westphalia, Germany

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