Microchimeric Cell Tranfer From Mother to Child - Pilo Study

• Conditions: Breastfeeding

• Registration Number: NCT05250219
• Lead Sponsor: University Hospital, Montpellier

Brief Summary

Microchimeric cells are known to be transferred from mother to child through the placenta. However it has been postulated that they could also be transferred through breastfeeding.

This study is designed to analyse lymphoid and digestive tract tissues (tonsils and appendixes) in male infants (or young adults) to research microchimeric female cells and try to correlate the frequency and localisation of said cells to the breastfeeding status during early infancy

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2018-12-24
• Study Start: 2021-09-01
• Status Verified: 2022-02
• Study First Submitted QC: 2022-02-10
• Last Update Submitted: 2022-02-10
• Study First Posted: 2022-02-22
• Last Update Posted: 2022-02-22
• Primary Completion: 2022-12-01
• Study Completion: 2022-12-31

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Male
• Target Recruitment: 80

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
to determine Quantifying the microchimeric cells	day 1	Quantifying the microchimeric cells : Cell quantification will be assessed through FISH staining (automated cell counting) and qPCR
To identify microchimeric cells of maternal origin in digestive tissues	day 1	To identify microchimeric cells of maternal origin in digestive tissues in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
to determine locating the cells in tissues	day 1	locating the cells in tissues : Appendix histology slides will be analysed after FISH staining to locate the microchimeric cells
To identify microchimeric cells of maternal origin in lymphoid	day 1	To identify microchimeric cells of maternal origin in lymphoid in young males cytogenetic FISH staining, coupled with MS-PCR, to identify microchimeric cells.
to determine trying to find a correlation between breastfeeding status	day 1	trying to find a correlation between breastfeeding status : Microchimeric cell quantification will be confronted to the breastfeeding status in infancy (collected during patient inclusion) to validate a correlation

Trial Locations

Locations (1)

Uh Montpellier; 🇫🇷 Montpellier, France