Exercise-Induced Gut Permeability in Normal-weight Obesity
- Conditions
- Normal-weight ObesityObesity
- Interventions
- Other: Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max)
- Registration Number
- NCT05889767
- Lead Sponsor
- Oklahoma State University
- Brief Summary
Exercise acutely increases gut permeability and inflammation, even in healthy populations. However, whether this response differs in groups at-risk for CVD that present with low-grade inflammation (e.g., normal-weight obesity) has yet to be examined. The investigators aim to measure serum indicators of gut permeability in those with normal-weight obesity pre- and post-short, intense exercise and sustained, moderate exercise
- Detailed Description
The investigator's overall goal is to examine how indicators of gut permeability change in response to exercise in individuals with normal-weight obesity (i.e., normal body mass index \[BMI\] and high body fat percent) relative to those with a normal BMI and low body fat percent and those with a BMI in the obesity range and high body fat percent.
The investigators will examine indicators of gut permeability (specified in primary outcomes) in response to a short, intense bout of exercise (i.e., VO2 max test on a cycle ergometer) and sustained, moderate bout of exercise (i.e., 45 minutes at 65% measured VO2 max on a cycle ergometer).
Additionally, the investigators will measure basic anthropometrics, blood lipids and glucose, and body composition with DXA.
Recruitment & Eligibility
- Status
- RECRUITING
- Sex
- All
- Target Recruitment
- 30
Control group: normal BMI, body fat percentage < 25% (male) or < 35% (female)
Normal-weight obesity: normal BMI, body fat percentage > 25% (male) or > 35% (female)
Overt obesity: BMI in obesity range, body fat percentage > 25% (male) or > 35% (female)
- Presence of pacemaker
- Pregnant
- Postmenopausal status
- History of chronically using of tobacco products, illicit drugs, anti-inflammatory drugs (e.g., NSAIDs), lipid lowering drugs
- Recent use of antibiotics (< 6 weeks) and NSAIDs (< 3 days)
- Established cardiometabolic disease (e.g., cardiovascular disease, type 2 diabetes) diseases inflammatory in nature (e.g., rheumatoid arthritis, inflammatory bowel disease), and/or diagnosed irritable bowel syndrome.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Arm && Interventions
Group Intervention Description Normal-weight obesity Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max) Individuals with normal-weight obesity will be defined as having normal BMI (18.5 - 24.9 kg/m2), body fat percent \> 25% (male) or \> 35% (female). Overt obesity and high body fat percent Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max) Overt obesity (BMI \> 30 kg/m2) with high body fat percent (\> 25% \[male\] or \> 35% \[female\]) will be used as a high-risk comparator group. Normal weight and low body fat percent Exercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max) The low risk comparator group for this study will consist of individuals with normal BMI (18.5 - 24.9 kg/m2) and body fat percent \< 25% (male) or \< 35% (female).
- Primary Outcome Measures
Name Time Method Serum concentrations of FABP2 following short, intense exercise Approximately 8 months The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Serum concentrations of IL-6 following sustained, moderate exercise Approximately 8 months The investigators will examine peak and absolute change in this inflammatory marker after 45-minutes of exercise at 65% VO2 max on a cycle ergometer.
Serum concentrations of LBP following short, intense exercise Approximately 8 months The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Serum concentrations of sCD14 following short, intense exercise Approximately 8 months The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer.
Serum concentrations of FABP2 following sustained, moderate exercise Approximately 8 months The investigators will examine peak and absolute change in this indicator of gut permeability after 45-minutes of exercise at 65% VO2 max on a cycle ergometer.
- Secondary Outcome Measures
Name Time Method VO2 Max Approximately 8 months The investigators will compare VO2 max after the short, intense exercise session across study groups.
Heart rate dynamics during exercise sessions Approximately 8 months The investigators will compare heart rate dynamics during the short, intense and moderate, sustained exercise session across study groups.
Respiratory exchange ratio (RER) during VO2 max test Approximately 8 months The investigators will compare RER dynamics during the short, intense exercise session across study groups.
Maximum workload during exercise sessions Approximately 8 months The investigators will compare workload dynamics in watts during the short, intense and moderate, sustained exercise session across study groups.
Trial Locations
- Locations (1)
208 Nancy Randolph Davis, Oklahoma State University
🇺🇸Stillwater, Oklahoma, United States