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Evaluation of Diagnostic Performance of VisioCyt® Test, in Case of Suspicion of Urothelial Bladder Tumors

Not Applicable
Completed
Conditions
Bladder Cancer
Interventions
Other: VisioCyt® diagnostic test
Registration Number
NCT02966691
Lead Sponsor
Vitadx
Brief Summary

This clinical study is designed to evaluate the diagnostic performance of VisioCyt test, which would improve the early diagnosis of bladder cancer in order to optimize the management of individual patients as part of a cancer diagnostic monitoring. The diagnostic method of VisioCyt® device is based on the analysis by transmission and fluorescence, of urinary cytology slides prepared according to the protocol VisioCyt.

Detailed Description

This clinical trial is divided into two main phases:

I. The first one involves the construction of better image processing algorithm from a learning sample. The learning sample will include 600 virtual slides made from the urine sample of the first 600 patients enrolled.

II. The second one, will evaluate from an evaluation sample, the diagnostic performance of the test VisioCyt, to identify patients with versus without bladder tumors, using as comparator the results of the examination of reference: histology. The evaluation sample will include 400 slides, made from the urine sample of the last 400 patients enrolled.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
1360
Inclusion Criteria
  • Age ≥18 years

  • Patient affiliated to a social security system

  • Patient able to understand the protocol.

  • Signature of patient consent form before the start of the study

  • Negative urine culture

  • Patient for which is programmed a bladder Endoscopy for :

    • suspected bladder cancer (de novo, monitoring or relapse)
    • Exploration lower urinary tract excluding a suspected bladder cancer or prostate cancer
Exclusion Criteria
  • Age <18 years
  • Ongoing Urinary tract infection untreated
  • Private person of liberty or under guardianship (including guardianship)
  • Bladder cancer excluding urothelial carcinoma
  • Carcinoma of high urinary tract, associated
  • Lithiasis pathology
  • Patient who underwent renal transplantation

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Patients 'sick'VisioCyt® diagnostic testThe patients of this arm have a clinical signs of bladder cancer with : * a positive result of bladder endoscopy * or an negative endoscopy and a positive result of the conventional cytology The intervention consists to collect an additional urine sample (50 ml), the day of the bladder endoscopy, which is performed during the current practice .This urine sample will be used for the preparation of cytology slides according to the Visiocyt protocol, in order to be digitized
Patients 'healthy'VisioCyt® diagnostic testThe patients of this arm have no suspicion of bladder cancer with negative results of their bladder endoscopy and conventional cytology. The intervention consists to collect an additional urine sample (50 ml), the day of the bladder endoscopy, which is performed during the current practice . This urine sample will be used for the preparation of cytology slides according to the Visiocyt protocol, in order to be digitized.
Patients 'monitoring'VisioCyt® diagnostic testThe patients of this arm have a history of bladder cancer, but the results of their follow up examinations (cytologic and endoscopic) are negative (no tumor). The intervention consists to collect an additional urine sample (50 ml), the day of the bladder endoscopy, which is performed during the current practice . This urine sample will be used for the preparation of cytology slides according to the Visiocyt protocol, in order to be digitized.
Primary Outcome Measures
NameTimeMethod
Identification and classification of cells with the VisioCyt test to create an image processing algorithm9 months

\* Identification and classification of cells, taking the Paris System in 2016 as a referential

Identification of presence or absence of a membrane fluorescence perished with the VisioCyt test to create an image processing algorithm9 months

presence or absence of a membrane fluorescence perished, on slides

sensitivity of urinalysis by VisioCyt testthrough the study completion

number of patients "declared positive" with the VisioCyt test among the patients actually suffering from bladder carcinoma

specificity of urinalysis by VisioCyt testthrough the study completion

number of patients "declared negative" with the VisioCyt test among the really healthy patients

Secondary Outcome Measures
NameTimeMethod
sensitivity of urinalysis by VisioCyt test according to the cell grade of the patientsthrough the study completion

number of patients "declared positive" with the VisioCyt test among the patients with a "low grade" or "high grade" bladder carcinoma

sensitivity of urinalysis by VisioCyt test according to medical history of the patientsthrough the study completion

number of patients "declared positive" with the VisioCyt test among the patients with a first diagnosis of bladder carcinoma or among patients with a previous bladder carcinoma under monitoring

comparison of the sensitivity of the urinalysis by VisioCyt test versus conventional urine cytologythrough the study completion

number of patients "declared positive" with the VisioCyt test versus patients "declared positive" with the conventional urine cytology

comparison of the specificity urinalysis by VisioCyt test versus conventional urine cytologythrough the study completion

number of patients "declared negative" with the VisioCyt test versus patients "declared negative" with the conventional urine cytology

Trial Locations

Locations (14)

Hôpital d'Antony

🇫🇷

Antony, France

Hôtel Dieu, CHR de Nantes

🇫🇷

Nantes, France

Hôpital Edouard Herriot

🇫🇷

Lyon, France

Institut Paoli-Calmettes

🇫🇷

Marseille, France

Clinique BeauSoleil

🇫🇷

Montpellier, France

CHU Caremeau, Nimes

🇫🇷

Nimes, France

CHRU Nancy

🇫🇷

Vandœuvre-lès-Nancy, France

Clinique " la Châtaigneraie "

🇫🇷

Beaumont, France

Hopital Rangueil

🇫🇷

Toulouse, France

CHU de Besançon

🇫🇷

Besançon, France

CHU Rennes

🇫🇷

Rennes, France

Hôpital Foch

🇫🇷

Suresnes, France

CHU de Clermont-Ferrand

🇫🇷

Clermont-Ferrand, France

Hôpital Cochin

🇫🇷

Paris, France

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