MedPath

Evaluation of High Dose Methotrexate Toxicity

Not yet recruiting
Conditions
Methotrexate Toxicity
Registration Number
NCT06123403
Lead Sponsor
Sohag University
Brief Summary

This study aims to:

1. Evaluation of high dose methotrexate related toxicities among children admitted to Sohag Oncology Centre.

2. Role of methotrexate blood level in prediction of high dose methotrexate related toxicities.

3. Role of Cystatin C as a predictor for acute kidney injury in high dose methotrexate related toxicities.

4. Factors affecting outcome of high dose methotrexate related toxicities.

Detailed Description

It is a prospective observational study will be carried out on children receiving high dose methotrexate in Sohag Oncology Centre with one-year observation starting from January 2024.

All children receiving high dose methotrexate in Sohag Oncology Centre and fulfill the study inclusion criteria with one-year observation starting from January 2024. Informed consent will be taken from the parents/guardians.

A Prepared sheet to collect data related to this study that include:

1. Socio-demographic data: e.g. age, sex, residence in addition to dose of MTX and tumor type.

2. General and systemic manifestations: gastrointestinal manifestations, neurological manifestations, respiratory and hematological manifestations.

3. Investigations: blood samples will be collected for CBC, Urea, Creatinine, ALT, AST, Methotrexate level and Cystatin C.

4. Treatment lines: fluid, folinic acid, alkalinization of urine, blood elements, hemodialysis and colony stimulating factors.

5. Outcome: complete recovery, residual effects, complications or death.

Recruitment & Eligibility

Status
NOT_YET_RECRUITING
Sex
All
Target Recruitment
100
Inclusion Criteria
  • Children admitted to Sohag Oncology Centre, age from 1 to 18 years
  • receive high dose methotrexate therapy equal to or more than 1 gm/m2 surface area.
Exclusion Criteria
  • WBCs less than 1000 / microliter
  • Platelet count less than 50000 / microliter
  • Renal affection
  • Hepatic affection
  • Respiratory affection
  • Diabetes mellitus
  • Cardiovascular disease

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Role of Cystatin C in prediction of acute kidney injuryBaseline

Venous samples will be collected about 3 milliliters under complete aseptic condition then centrifuged then the level of Cystatin C will be measured and will be used as abiomarker to detect acute kidney injury associated with high dose methotrexate related toxicities We will see if the test could predict occurrence of acute kidney injury before it happens or not

Role of methotrexate blood level in prediction of methotrexate related toxicitiesbaseline

Venous blood will be collected under complete aseptic condition about 5 milliliters to measure serum methotrexate level using drug analyzer then the level of methotrexate will be used as apredictor to identify high dose methotrexate related toxicities in pediatric malignancies We will see if the test could predict the occurrence of toxicities before it happens or not

Secondary Outcome Measures
NameTimeMethod
© Copyright 2025. All Rights Reserved by MedPath