A Phase 1 Randomized, Double-Blind, Placebo Controlled Clinical Trial to Evaluate the Safety and Immunogenicity of 3 Different HIV-1 DNA Priming Regimens (Nat-B Env, CON-S Env, and Mosaic Env) With MVA-CMDR Boosts in Healthy, HIV-1-Uninfected Adults
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 入组人数
- 105
- 试验地点
- 14
- 主要终点
- Laboratory measure of safety: measurement of white blood cells (WBC)
研究概览
简要总结
The purpose of this study is to evaluate the safety and immune response to three DNA vaccines and a MVA-CMDR vaccine that may boost the immune response to the DNA vaccines in healthy, HIV-uninfected adults.
详细描述
This study will evaluate the safety, tolerability, and immunogenicity to four different HIV vaccines in healthy, HIV-uninfected adults. The vaccines include three DNA vaccines-DNA Nat-B env, DNA CON-S env, and DNA Mosaic env-and a vaccine called MVA-CMDR that may boost the immune response to the DNA vaccines.
The study will enroll healthy, HIV-uninfected participants aged 18 to 50 years. Participants will be randomly assigned to one of three groups and will receive either one of the experimental vaccine regimens or a placebo vaccine regimen. Group 1 participants will receive the DNA Nat-B env and MVA-CMDR vaccines or placebo. Group 2 participants will receive the DNA CON-S env and MVA-CMDR vaccines or placebo. Group 3 participants will receive DNA Mosaic env and MVA-CMDR vaccines or placebo.
All participants will receive one of their assigned vaccines at study entry (Month 0), and Months 1, 2, 4, and 8.
Total study duration will be either 3 years after enrollment (for participants in the United States) or 5 years after enrollment (for participants in Switzerland). For all participants, study visits will occur at study entry (Month 0), and Months 0.5, 1, 1.5, 2, 2.5, 4, 4.5, 8, 8.25, 8.5, 11, 13.75, and 14. After the last study visit, participants will be contacted annually by phone or e-mail for a total of 3 (U.S. participants) or 5 (Switzerland participants) years to answer questions about their health.
All study visits will include a physical exam, HIV risk reduction counseling, and an interview and/or questionnaire. Select study visits will include blood collection, urine and stool collection, HIV testing, an electrocardiogram (ECG), and a pregnancy test for participants who were born female. For participants receiving the MVA-CMDR vaccine, select study visits may also include an assessment of cardiac symptoms.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Prevention
- 盲法
- Quadruple (Participant, Care Provider, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 50 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •General and Demographic Criteria
- •Access to a participating HIV Vaccine Trials Network (HVTN) clinical research site (CRS) and willingness to be followed for the planned duration of the study
- •Ability and willingness to provide informed consent
- •Assessment of understanding: participant demonstrates understanding of this study; completes a questionnaire prior to first vaccination with verbal demonstration of understanding of all questionnaire items answered incorrectly
- •Willing to be contacted annually after completion of scheduled clinic visits for a total of 3 years for U.S. participants (5 years for participants in Switzerland) following initial study injection
- •Agrees not to enroll in another study of an investigational research agent
- •Good general health as shown by medical history, physical exam, and screening laboratory tests
- •HIV-Related Criteria
- •Willingness to receive HIV test results
- •Willingness to discuss HIV infection risks and amenable to HIV risk reduction counseling
- •Assessed by the clinic staff as being at "low risk" for HIV infection and committed to maintaining behavior consistent with low risk of HIV exposure through the last required protocol clinic visit
- •Laboratory Inclusion Values
- •Hemogram/Complete Blood Count (CBC)
- •Hemoglobin greater than or equal to 12.5 g/dL for participants who were born female, greater than or equal to 13.5 g/dL for participants who were born male
- •White blood cell count equal to 3,300 to 12,000 cells/mm^3
- •Total lymphocyte count greater than or equal to 800 cells/mm^3
- •Remaining differential either within institutional normal range or with site physician approval
- •Platelets equal to 125,000 to 550,000/mm^3
- •Chemistry panel: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase less than 1.25 times the institutional upper limit of normal; creatinine less than or equal to the institutional upper limit of normal
- •Cardiac Troponin T or I (cTnT or cTnI) does not exceed the institutional upper limit of normal
- •Negative HIV-1 and -2 blood test: U.S. participants must have a negative Food and Drug Administration (FDA)-approved enzyme immunoassay (EIA). Non-U.S. site may use locally available assays that have been approved by HVTN Laboratory Operations.
- •Negative hepatitis B surface antigen (HBsAg)
- •Negative anti-hepatitis C virus antibodies (anti-HCV), or negative HCV polymerase chain reaction (PCR) if the anti-HCV is positive
- •Normal urine:
- •Negative urine glucose, and
- •Negative or trace urine protein, and
- •Negative or trace urine hemoglobin (if trace hemoglobin is present on dipstick, a microscopic urinalysis with red blood cells levels within institutional normal range)
- •Reproductive Status
- •Participants who were born female: negative serum or urine beta human chorionic gonadotropin (beta-HCG) pregnancy test performed prior to vaccination on the day of initial vaccination. Persons who are NOT of reproductive potential due to having undergone total hysterectomy or bilateral oophorectomy (verified by medical records), are not required to undergo pregnancy testing.
- •Reproductive status: participants who were born female must:
- •Agree to consistently use effective contraception (information on effective contraception methods can be found in the protocol) for sexual activity that could lead to pregnancy from at least 21 days prior to enrollment through the last required protocol clinic visit;
- •Or not be of reproductive potential, such as having reached menopause (no menses for 1 year) or having undergone hysterectomy, bilateral oophorectomy, or tubal ligation;
- •Or be sexually abstinent.
- •Participants who were born female must also agree not to seek pregnancy through alternative methods, such as artificial insemination or in vitro fertilization until after the last required protocol clinic visit.
排除标准
- •Blood products received within 120 days before first vaccination
- •Investigational research agents received within 30 days before first vaccination
- •Body mass index (BMI) greater than or equal to 40
- •Intent to participate in another study of an investigational research agent during the planned duration of the HVTN 106 study
- •Pregnant or breastfeeding
- •Vaccines and Other Injections
- •Smallpox vaccine received within the last 5 years
- •HIV vaccine(s) received in a prior HIV vaccine trial. For participants who have received control/placebo in an HIV vaccine trial, the HVTN 106 Protocol Safety Review Team (PSRT) will determine eligibility on a case-by-case basis and the identity of the study control/placebo must be obtained.
- •Non-HIV experimental vaccine(s) received within the last 5 years in a prior vaccine trial. Exceptions may be made for vaccines that have subsequently undergone licensure by the FDA. For participants who have received control/placebo in an experimental vaccine trial, the HVTN 106 PSRT will determine eligibility on a case-by-case basis. For participants who have received an experimental vaccine(s) more than 5 years ago, eligibility for enrollment will be determined by the HVTN 106 PSRT on a case-by-case basis.
- •Live attenuated vaccines other than influenza vaccine received within 30 days before first vaccination or scheduled within 14 days after injection (e.g., measles, mumps, and rubella [MMR]; oral polio vaccine [OPV]; varicella; yellow fever)
- •Influenza vaccine or any vaccines that are not live attenuated vaccines and were received within 14 days prior to first vaccination (e.g., tetanus, pneumococcal, hepatitis A or B)
- •Allergy treatment with antigen injections within 30 days before first vaccination or that are scheduled within 14 days after first vaccination
- •Immune System
- •Immunosuppressive medications received within 168 days before first vaccination. (Not excluded: [1] corticosteroid nasal spray; [2] inhaled corticosteroids; [3] topical corticosteroids for mild, uncomplicated dermatitis; or [4] a single course of oral/parenteral corticosteroids at doses less than 2 mg/kg/day and length of therapy less than 11 days with completion at least 30 days prior to enrollment
- •Serious adverse reactions to vaccines or to vaccine components such as neomycin or streptomycin, including anaphylaxis and related symptoms such as hives, respiratory difficulty, angioedema, and/or abdominal pain. (Not excluded: a participant who had a nonanaphylactic adverse reaction to pertussis vaccine as a child)
- •Immunoglobulin received within 60 days before first vaccination
- •Autoimmune disease
- •Immunodeficiency
- •Hypersensitivity to eggs and/or egg products
- •Clinically Significant Medical Conditions
- •Untreated or incompletely treated syphilis infection
- •Clinically significant medical condition, physical examination findings, clinically significant abnormal laboratory results, or past medical history with clinically significant implications for current health. More information on this criterion can be found in the protocol.
- •Any medical, psychiatric, occupational, or other condition that, in the judgment of the investigator, would interfere with, or serve as a contraindication to, protocol adherence, assessment of safety or reactogenicity, or a participant's ability to give informed consent
- •Psychiatric condition that precludes compliance with the protocol. Specifically excluded are persons with psychoses within the past 3 years, ongoing risk for suicide, or history of suicide attempt or gesture within the past 3 years.
- •Current anti-tuberculosis (TB) prophylaxis or therapy
- •Asthma exclusion criteria: Asthma other than mild, well-controlled asthma. More information on this criterion can be found in the protocol.
- •Diabetes mellitus type 1 or type 2, including cases controlled with diet alone (Not excluded: history of isolated gestational diabetes)
- •Thyroidectomy, or thyroid disease requiring medication during the last 12 months
- •Hypertension:
- •If a person has been found to have elevated blood pressure or hypertension during screening or previously, exclude for blood pressure that is not well controlled. Well-controlled blood pressure is defined as consistently less than or equal to 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic, with or without medication, with only isolated, brief instances of higher readings, which must be less than or equal to 150 mm Hg systolic and less than or equal to 100 mm Hg diastolic. For these participants, blood pressure must be less than or equal to 140 mm Hg systolic and less than or equal to 90 mm Hg diastolic at enrollment.
- •If a person has NOT been found to have elevated blood pressure or hypertension during screening or previously, exclude for systolic blood pressure greater than or equal to 150 mm Hg at enrollment or diastolic blood pressure greater than or equal to 100 mm Hg at enrollment.
- •History of myocarditis, pericarditis, cardiomyopathy, congestive heart failure with permanent sequelae, clinically significant arrhythmia (including any arrhythmia requiring medication, treatment, or clinical follow-up)
- •Participants who have 2 or more of the following cardiac risk factors:
- •Participant report of history of elevated blood cholesterol defined as fasting low-density lipoprotein (LDL) greater than 160 mg/dL;
- •First degree relative (e.g., mother, father, brother, or sister) who had coronary artery disease before the age of 50 years;
- •Current smoker; or
- •BMI greater than or equal to 35
- •Electrocardiogram (ECG) with clinically significant findings, or features that would interfere with the assessment of myo/pericarditis, as determined by the contract ECG Lab, cardiologist, or study clinician. More information on this criterion can be found in the protocol.
- •History of hereditary angioedema, acquired angioedema, or idiopathic angioedema
- •Bleeding disorder diagnosed by a doctor (e.g., factor deficiency, coagulopathy, or platelet disorder requiring special precautions)
- •Malignancy (Not excluded: participant who has had malignancy excised surgically and who, in the investigator's estimation, has a reasonable assurance of sustained cure or who is unlikely to experience recurrence of malignancy during the period of the study)
- •Seizure disorder: History of seizure(s) within past 3 years. Also exclude if participant has used medications in order to prevent or treat seizure(s) at any time within the past 3 years.
- •Asplenia: any condition resulting in the absence of a functional spleen
结局指标
主要结局
Laboratory measure of safety: measurement of white blood cells (WBC)
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of platelets
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of creatinine
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of neutrophils
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of alanine aminotransferase (ALT)
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of aspartate aminotransferase (AST)
时间窗: Measured through Month 8
Severity of local and systemic injection site reactogenicity signs and symptoms for each type of vaccine
时间窗: Measured through Month 8
Number of participants with early discontinuation of vaccinations and reason for discontinuation
时间窗: Measured through Month 8
Frequency of local and systemic injection site reactogenicity signs and symptoms for each type of vaccine
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of lymphocytes
时间窗: Measured through Month 8
Frequency of adverse events (AEs)
时间窗: Measured through participant follow-up (3 and 5 years for participants in the United States and Switzerland, respectively)
Categorized by MedDRA body system, MedDRA preferred term, severity, and assessed relationship to study products; detailed description of all AEs meeting Division of AIDS (DAIDS) criteria for expedited reporting (EAE)
Laboratory measure of safety: measurement of hemoglobin
时间窗: Measured through Month 8
Laboratory measure of safety: measurement of alkaline phosphatase
时间窗: Measured through Month 8
次要结局
- Response rate of CD8 T-cell responses 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Total magnitude of CD8 T-cell responses 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Response rate of CD4 T-cell responses 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Total magnitude of CD4 T-cell responses 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Total magnitude of CD4 T-cell responses 2 weeks after each MVA vaccination(Measured through Month 8.5)
- Breadth of HIV-specific binding IgG Env Ab responses 2 weeks after the last MVA vaccination(Measured through Month 8.5)
- Breadth of serum nAbs to a panel of standardized HIV-1 isolates(Measured through Month 14)
- Breadth of CD4 T-cell responses determined by epitope mapping, 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Breadth of CD8 T-cell responses determined by epitope mapping, 2 weeks after the last DNA vaccination(Measured through Month 2.5)
- Breadth of CD4 T-cell responses determined by epitope mapping, 2 weeks after MVA vaccination(Measured through Month 8.5)
- Response rate of CD8 T-cell responses 2 weeks after each MVA vaccination(Measured through Month 8.5)
- Total magnitude of CD8 T-cell responses 2 weeks after each MVA vaccination(Measured through Month 8.5)
- Breadth of CD8 T-cell responses determined by epitope mapping, 2 weeks after MVA vaccination(Measured through Month 8.5)
- Magnitude of HIV-specific binding immunoglobulin A (IgA) Env Ab responses 2 weeks after the last MVA vaccination(Measured through Month 8.5)
- Response rate of CD4 T-cell responses 2 weeks after each MVA vaccination(Measured through Month 8.5)
- Magnitude of HIV-specific binding immunoglobulin G (IgG) Env antibody (Ab) responses 2 weeks after the last MVA vaccination(Measured through Month 8.5)
- Breadth of HIV-specific binding IgA Env Ab responses 2 weeks after the last MVA vaccination(Measured through Month 8.5)
- Magnitude of serum neutralizing antibodies (nAbs) to a panel of standardized HIV-1 isolates(Measured through Month 14)