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A Prospective Multicenter Clinical Trial of MRD-based Treatment Strategy in Children and Young Adults With AML

Phase 3
Recruiting
Conditions
Acute Myeloid Leukemia, Childhood
Interventions
Other: HSCT
Registration Number
NCT03846362
Lead Sponsor
Federal Research Institute of Pediatric Hematology, Oncology and Immunology
Brief Summary

Minimal-residual disease (MRD) will be measured either by flow cytometry, or polymerase chain reaction (PCR) methods, in 3 check-points and it will be one of the decision-making control parameter for the optimal therapy tactics.

Patients with initially high-risk group and those with high MRD after 2 initial courses of chemotherapy will be assigned to the allogenic transplantation of the hematopoietic stem cells from Human Leucocyte Antigen (HLA) matched or haploidentical family donors.

Detailed Description

Genetic alterations in acute myeloid leukemia (AML) clone are well known prognostic risk factors of AML relapse. Standard risk group includes favorable t (15;17) (q22; q21) and inv (16)/t (16;16). High-risk patients have a complex karyotype rearrangement (3 and more), inversion of the long arm in 3rd chromosome and EVI1 gene rearrangement, monosomy 5 and 7, translocations involving KMT2A gene and several rare translocations. All other genotype alterations attributed to the moderate risk group.

Besides genetic factors, detection of the minimal residual disease (MRD) after initial chemotherapy and its decrease rate after 1st postremission chemotherapy with high dose Cytarabine and anthracyclines, plays a crucial role in the development of the morphologic relapse. Patients with PCR-MRD\<0,1% after 2 courses of chemotherapy have a 30% or less risk of relapse, while PCR-MRD\>0,1% - over 70%. In the clinical trial investigators are planning to measure MRD either by immune-phenotype, or PCR methods, in 3 check-points and it will be one of decision-making control parameter for the optimal therapy tactics.

Patients with initially high-risk group and those with high MRD after 2 initial courses of chemotherapy will be assigned to the allogenic transplantation of the hematopoietic stem cells from HLA- matched or haploidentical family donors.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
500
Inclusion Criteria
  1. de novo acute myeloid leukemia
  2. signed informed consent
Exclusion Criteria

diagnosis of: Fanconi anemia, acute promyelocytic leukemia, MDS, JMML, AML as secondary malignancy, Dawn syndrome.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
intermediate risk MRD2>0,1%HSCTMRD2\>0,1% - FLA - MRD3 - HSCT
Primary Outcome Measures
NameTimeMethod
relapse-free survival (RFS)1 year

relapse-free survival from date of diagnosis till date of relapse, or date of death (whichever comes first) or date of last follow up

Secondary Outcome Measures
NameTimeMethod
overall survival (OS)1 year
event-free survival (EFS)2 years

Event=relapse/nonresponse, death or second malignancy

The proportion of of patients with severe adverse effects6 months

The proportion of of patients with severe adverse effects of therapy according to CTCAE (ver 4.3)

The proportion of of patients with severe infections1 month

The proportion of of patients with severe infections: number of episodes, grade, after each course of chemotherapy

The proportion of of patients with severe cardiotoxicity1 year

The proportion of of patients with severe cardiotoxicity: number of episodes and %EF by echocardiogam

MRD dynamic1 months

MRD (IFT and/or PCR) dynamic between check-points

MRD specificity and sensitivity1, 2, 3 months

MRD specificity and sensitivity in relapse prognosis

Cumulative incidence of relapse6 months, 1 year

competing event - death in CR

Cumulative incidence of transplant-related mortality6 months after HSCT

for transplanted patients

Cumulative incidence of aGvHD II-IV grade100 days after HSCT

for transplanted patients

Cumulative incidence of cGvHD1 year after HSCT

for transplanted patients

Trial Locations

Locations (2)

Dmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology

🇷🇺

Moscow, Russian Federation

Regional Children's Clinical Hospital â„– 1

🇷🇺

Ekaterinburg, Sverdlovsk Oblast, Russian Federation

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