Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 55
- 主要终点
- Estimation of lesional ERAP1 in psoriatic skin
研究概览
简要总结
A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls.
Skin biopsy from patients (psoriasis lesional and non lesional) & control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis & PCR.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- Double (Participant, Investigator)
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •patients with psoriasis vulgaris
- •patients over 18 years of age
- •both sexes
排除标准
- •patients on treatment for psoriasis within the last two months
- •assoiciated autoimmune diseases eg SLE
- •pregnancy
研究组 & 干预措施
cases of psoriasis
group of 25 cases of psoriasis subjected to skin biopsy for detection of lesional and non lesional expression of ERAP1 by RT-PCR
干预措施: RT-PCR for ERAP1 detection (Diagnostic Test)
normal controls
group of 30 age and sex matched healthy controls o subjected to skin biopsy from normal skin for detection of expression of ERAP1 by RT-PCR
干预措施: RT-PCR for ERAP1 detection (Diagnostic Test)
结局指标
主要结局
Estimation of lesional ERAP1 in psoriatic skin
时间窗: Maximum 2 years
DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT-
次要结局
- Estimation of non lesional ERAP1 in psoriatic skin(Maximum 2 years)
研究者
Rana F Hilal, MD
Lecturer of dermatology
Kasr El Aini Hospital