Effects of Single Plasma Exchange and Double Filtration Plasmapheresis (DFPP) on Peripheral Lymphocyte Phenotypes in Patients With Chronic Inflammatory Demyelinating Polyradiculoneuropathy

Early Phase 1

Completed

• Conditions: Polyradiculoneuropathy, Chronic Inflammatory Demyelinating
• Interventions: Biological: Blood sampling

• Registration Number: NCT04742374
• Lead Sponsor: Centre Hospitalier Universitaire de Nīmes

Brief Summary

CIDP, a rare disorder affecting young adults, causes gradual weakness of the limbs, areflexia and impaired sensory function. New CIDP phenotypes without antibodies but with modified cell profiles have been described. Treatments include corticotherapy, IVIg and plasmapheresis but the latter's action mechanisms remain unclear. Plasmapheresis supposedly removes toxic agents like antibodies from plasma but it is uncertain whether it has an immune-modulating effect. Also, the refining mechanisms of the two main plasmapheresis techniques - single plasma exchange and double filtration plasmapheresis (DFPP) - are different and unclear. This study aims to compare the evolution of peripheral lymphocyte profiles in patients with CIDP according to their treatment (single centrifugation plasmapheresis or DFPP) to better grasp the action mechanisms of both techniques.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2021-01-26
• Study Start: 2021-02-01
• Study First Submitted QC: 2021-02-03
• Study First Posted: 2021-02-08
• Primary Completion: 2022-02-03
• Study Completion: 2022-02-03
• Status Verified: 2022-07
• Last Update Submitted: 2023-09-25
• Last Update Posted: 2023-09-28

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 8

Inclusion Criteria

• Patients with confirmed Chronic Inflammatory Demyelinating Polyneuropathy (CIDP) without pathogenic antibodies and stable under therapeutic plasmapheresis with one session every 2-3 weeks for at least two months.
• Patients who have given free written informed consent.
• Patients who have signed the consent form.
• Patients affiliated to or beneficiaries of a health insurance scheme.
• Adult patients (≥18 ans).
• Healthy subjects of the same age and sex

Exclusion Criteria

• Patients Under the age of 18.
• Patients under immunosuppressive treatment for less than 3 months.
• Patients participating in Category 1 research involving human subjects.
• Patients in an exclusion period determined by another study.
• Patients under legal protection, guardianship or curatorship.
• Patients unable to express consent.
• Patients for whom it is impossible to give informed information.
• Pregnant, parturient or breastfeeding patients.
• Healthy subject with chronic pathology or drug treatment

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Group B	Blood sampling	Patients alternating double filtration plasmapheresis followed by single plasma exchange etc.
Group A	Blood sampling	Patients alternating single plasma exchange followed by double filtration plasmapheresis etc.

Primary Outcome Measures

Name	Time	Method
Cycle 2, Group A: TH1 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 2, Group A: TH1 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 2, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 cells will be measured in %.
Cycle 1, Group A : T-reg cells BEFORE first single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group B : T17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 1, Group B : T-reg cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: TH17 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group B: TH17 cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 1, Group A : TH1 cells 7-10 days AFTER single plasma exchange	Day 7 -10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells 7 - 10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group B: TH1 cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 3, Group A: TH17 cells AFTER single plasma exchange	Day 1, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 1, Group A : T-reg cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group B : T-reg cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group A: TH17 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: T-reg cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 3, Group A: TH1 cells AFTER single plasma exchange	Day 1, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH1 cells BEFORE single plasma exchange	Day 0, Time 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH1 cells BEFORE first double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH1 cells 7-10 days AFTER the first double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH17 cells BEFORE the first double filtration plasmapheresis	Day0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 1, Group A : TH1 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells BEFORE first single plasma exchange	Day 0, Time 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : TH17 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group A : T-reg cells 7 - 10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 1, Group B : TH1 cells AFTER the first double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 1, Group B : TH17 cells AFTER the first double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group B: TH1 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 2, Group B: TH1 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 3, Group A: T1 cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group A : TH17 cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 2, Group A: T-reg cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 2, Group B: T17 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T17 cells will be measured in %.
Cycle 2, Group B: TH17 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T17 cells will be measured in %.
Cycle 2, Group B: T-reg cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 2, Group B: T-reg cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group A: T-reg cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group B: TH1 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: TH1 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: TH17 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group A: T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH1 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 2, Group B: T-reg cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group A: TH1 cells BEFORE single plasma exchange	Day 1, Hour 1	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T1 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : T17 cells 7-10 days AFTER single plasma exchange	Day 7 -10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group A : TH17 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group B : TH1 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : TH17 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 5, Group B : TH17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 3, Group B: TH17 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group A: TH1 cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: TH17 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 5, Group A : TH17 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group A : T-reg cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group B : TH1 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group A: TH17 cells BEFORE single plasma exchange	Day 1, Hour 1	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 3, Group A: T-reg cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group B: TH1 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group B: TH17 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group A: T-reg cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T-reg cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group B : T-reg cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : TH1 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group A : TH1 cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 3, Group A: TH17 cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 3, Group A: T-reg cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 3, Group B: TH17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 3, Group B: T-reg cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 3, Group B: T-reg cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 4, Group A: T1 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group A: TH17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH17 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 4, Group B : T-reg cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : TH17 cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 5, Group B : TH1 cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : TH17 cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 4, Group B : TH1 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH1 cells 7-10 days AFTER single plasma exchange	Day 7 -10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 4, Group B : TH17 cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 4, Group B : T-reg cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : TH1 cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group A : TH1 cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 cells will be measured in %.
Cycle 5, Group A : T-reg cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 5, Group A : T-reg cells 7-10 days AFTER single plasma exchange	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 6, Group A : TH1 cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : TH17 cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group A : TH1 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 5, Group B : T-reg cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 5, Group B : T-reg cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 5, Group B : T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group A : TH17 cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 6, Group A : T-reg cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group B : TH1 cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : T-reg cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 6, Group A : TH17 cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and the number of TH17 cells will be measured in %.
Cycle 6, Group B : TH1 cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : TH1 cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH1 peripheral lymphocyte cells will be measured in %.
Cycle 6, Group B : T-reg cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of T-reg cells will be measured in %.
Cycle 6, Group A : T-reg cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group A : T-reg cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and the number of T-reg cells will be measured in %.
Cycle 6, Group B : TH17 cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group B : TH17 cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of TH17 cells will be measured in %.
Cycle 6, Group B : T-reg cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and the number of T-reg cells will be measured in %.

Secondary Outcome Measures

Name	Time	Method
Cycle 1, Group B: CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T8 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CR6-CXCR3- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: B lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : B lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T4-DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B:T4 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 1, Group B: B lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : B lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 1, Group B: CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube 7-10 days AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) a,d measured as a percentage.
Cycle 2, Group A : B lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : B lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7 - 10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured a percentage.
Cycle 3, Group A : T8 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA-CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 2, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA-CCR7- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CC45RA+CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : B lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T4 lymphocytes 7 -10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 3, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes BEFORE plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes BEFORE plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes BEFORE plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0 Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	Day 0 Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 4, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells BEFORE single plasma exchange	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CD45RA-CCR7- cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : CCR6-CXCR3- cells AFTER single plasma exchange	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group A : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CCR6-CXCR3- cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 5, Group B : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells BEFORE double filtration plasmapheresis	Day 0, Hour 0	5 mL of blood will be collected in an EDTA tube BEFORE double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : B lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells AFTER double filtration plasmapheresis	Day 0, Hour 3	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T4 DR+ lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : T8 lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : B lymphocytes 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA+CCR7+ cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CD45RA-CCR7- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group A : CCR6-CXCR3- cells 7-10 days AFTER double filtration plasmapheresis	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER double filtration plasmapheresis and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells BEFORE single plasma exchange	Day 0, H0	5 mL of blood will be collected in an EDTA tube BEFORE single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA+CCR7+ cells AFTER single plasma exchange	Day 0, H3	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CCR6-CXCR3- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T4 DR+ lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : T8 lymphocytes 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7+ cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.
Cycle 6, Group B : CD45RA-CCR7- cells 7-10 days AFTER single plasma exchange	Day 7-10	5 mL of blood will be collected in an EDTA tube AFTER single plasma exchange and cell counts will be made by flow cytometry after marking with specific fluorescent antibodies (Luminex) and measured as a percentage.

Trial Locations

Locations (1)

Olivier MORANNE; 🇫🇷 Nîmes, France