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Antiplatelet Therapy Effect on Extracellular Vesicles in Acute Myocardial Infarction

Phase 4
Completed
Conditions
Myocardial Infarction
Interventions
Registration Number
NCT02931045
Lead Sponsor
Medical University of Warsaw
Brief Summary

Platelet activation and aggregation leads to myocardial infarction. Platelet P2Y12 receptors are essential for platelet activation. Antagonists against the P2Y12 receptor, which are established in secondary prevention of myocardial infarction, have unexplained anti-inflammatory effects. A novel P2Y12 receptor antagonist ticagrelor reduced infection-related mortality compared to clopidogrel, previous standard treatment for patients with myocardial infarction. Activated platelets release pro-inflammatory and procoagulant platelet extracellular vesicles. The investigators assume that decrease in infection-related mortality in patients treated with ticagrelor may be explained by greater inhibition of the release of platelet vesicles by ticagrelor, compared to clopidogrel. This study is expected to identify an additional mechanism of action of ticagrelor, which might contribute to the observed clinical benefits in patients treated with ticagrelor.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Age > 18 years
  • Informed consent to participate in the study
  • Percutaneous coronary intervention with stent implantation due to first S T elevation myocardial infarction, or first non S T -elevation myocardial infarction
  • Administration of a loading dose of clopidogrel
Exclusion Criteria
  • Known coagulopathy
  • Known history of bleeding disorder
  • Suspicion of intracranial haemorrhage
  • Need for oral anticoagulation therapy
  • Administration of glycoprotein (GP) II b - III a antagonists
  • Cardiogenic shock
  • Severe chronic renal failure (estimated glomerular filtration rate < 30 mL/min)
  • Severe liver insufficiency
  • Chronic dyspnea
  • Increased risk of bradycardia
  • Autoimmune disease
  • Infectious disease
  • Neoplasms
  • Pregnancy
  • Study drug intolerance
  • Co-administration of ticagrelor or clopidogrel with strong CYP3A4 inhibitors
  • Participation in any previous study with ticagrelor or clopidogrel

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
TicagrelorTicagrelorTicagrelor: oral, 180 mg once (loading dose) followed by 90 mg twice daily (maintenance dose)
ClopidogrelClopidogrelClopidogrel: oral, 300 mg or 600 mg once (loading dose) followed by 75 mg once daily (maintenance dose)
Primary Outcome Measures
NameTimeMethod
Concentration of Platelet Extracellular Vesicles/ml6 months following the beginning of antiplatelet therapy

Concentration of platelet extracellular vesicles/ml measured with flow cytometry

Secondary Outcome Measures
NameTimeMethod
Concentration of Extracellular Vesicles From Leukocytes6 months

Concentration of extracellular vesicles from leukocytes/ ml measured with flow cytometry

Concentration of Extracellular Vesicles Exposing Phosphatidylserine6 months

Concentration of extracellular vesicles exposing phosphatidylserine/ml measured with flow cytometry

Concentration of Extracellular Vesicles Exposing Fibrinogen6 months

Concentration of extracellular vesicles exposing fibrinogen/ ml measured with flow cytometry

Concentration of Extracellular Vesicles From Endothelial Cells6 months

The concentrations of extracellular vesicles from endothelial cells/ ml measured with flow cytometry

Trial Locations

Locations (2)

Laboratory of Experimental Clinical Chemistry, Academic Medical Centre of the University of Amsterdam

🇳🇱

Amsterdam, Netherlands

1st Chair and Department of Cardiology, Medical University of Warsaw

🇵🇱

Warsaw, Poland

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