Effects of Resistance Training Preconditioning on Skeletal Muscle Recovery From a Period of Disuse in Young Adults
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Atrophy, Disuse
- Sponsor
- Auburn University
- Enrollment
- 24
- Primary Endpoint
- Change in quadriceps strength via isokinetic dynamometry
- Status
- Not yet recruiting
- Last Updated
- 3 years ago
Overview
Brief Summary
The goal of this clinical trial is to compare the effects of resistance training (RT) preconditioning vs no training on disuse-induced atrophy and post-disuse resistance training in young healthy individuals. The main questions it aims to answer are:
- To determine if performing RT prior to a period of disuse enhances the regain of strength, skeletal muscle size, and skeletal muscle quality while performing RT after a period of disuse.
- To determine if performing RT prior to a period of disuse dampens the maladaptive effects of disuse on muscle size, muscle quality, and strength.
- To determine the anabolic and proteolytic mechanisms underpinning the observed outcomes.
Participants will:
- Perform either 6 weeks of resistance training or maintain an untrained lifestyle
- Perform 2 weeks of limb immobilization induced disuse of a randomized leg
- Perform 6 weeks of resistance training
Researchers will compare the resistance training preconditioning condition vs the non-trained condition to see if resistance training prior to a period of disuse is beneficial during the disuse period and in the return to training period on skeletal muscle size, strength, and underpinning molecular markers.
Investigators
Michael Roberts
Professor
Auburn University
Eligibility Criteria
Inclusion Criteria
- •Between the ages of 18-30
- •Free from medical implants in lower extremity that would interfere with low-dose radiation
- •Are not allergic to ultrasound gel or Lidocaine
- •Have no or minimal experience with resistance training (less than 1 training session/week for a 1 year period)
Exclusion Criteria
- •Have been exposed to medically necessary radiation in the past 12 months other than dental x-rays
- •Have known overt cardiovascular or metabolic disease
- •Have a medical condition that would contraindicate participating in an exercise program or donating a skeletal muscle biopsy (e.g. blood clotting disorder, taking blood thinners, etc.)
- •Are undergoing medical treatment for a disease that would interfere physiologically or logistically with study outcomes and/or protocols (e.g. undergoing chemotherapy and/or radiation for cancer treatment)
- •Have consumed supplemental protein, creatine, and/or agents that affect hormone status (e.g. testosterone, growth hormone boosters, etc.) within the previous 2 months.
Outcomes
Primary Outcomes
Change in quadriceps strength via isokinetic dynamometry
Time Frame: Through completion of study, 16 weeks
Strength of the quadriceps will be assessed via isokinetic dynamometry
Change in vastus lateralis fiber cross-sectional area
Time Frame: Through completion of study, 16 weeks
Upon the procurement and analysis of muscle from the vastus lateralis of participants, histological techniques will be used to assess fiber cross-sectional area of muscle fibers
Change in quadriceps strength via 3 repetition maximum testing
Time Frame: Through completion of study, 16 weeks
Strength of the quadriceps will be assessed via 3-repetition maximum testing of select exercises (leg press, leg extension, hex-bar deadlift)
Secondary Outcomes
- Change in activity of catabolic systems(Through completion of study, 16 weeks)
- Gene expression at the mRNA level of atrophy-associated genes (atrogenes)(Through completion of study, 16 weeks)
- Change in protein expression of anabolic signaling proteins(Through completion of study, 16 weeks)
- Change in protein expression of catabolic signaling proteins(Through completion of study, 16 weeks)