Alveolar Macrophage Programming Following Endotoxin Exposure
Trial Snapshot
- Phase
- Not Applicable
- Status
- Recruiting
- Sponsor
- National Jewish Health
- Enrollment
- 25
- Locations
- 1
- Primary Endpoint
- Resident alveolar macrophages and recruited macrophages will be distinguished from one another using multi-parameter flow cytometry, and the relative proportions enumerated
Study Overview
Brief Summary
The histologic hallmarks of lung inflammation include accumulation of inflammatory cells in the airspaces and interstitium, injury to alveolar epithelial and endothelial cells, loss of epithelial-capillary integrity and accumulation of edema fluid in the interstitium and airspaces. Accordingly, for alveolar repair to occur inflammation must be halted, debris and inflammatory cells removed, injured tissue cells replaced, and capillary barrier function re-established. Macrophages are key players in all of these. Here the investigators hypothesize that resident alveolar macrophages and recruited macrophages serve completely different functions, acting independently (i.e. division of labor) yet cooperatively (synergism).
Study Design
- Study Type
- Interventional
- Allocation
- Na
- Intervention Model
- Single Group
- Primary Purpose
- Basic Science
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 50 Years (Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Written, informed consent
Exclusion Criteria
- •Current or recent illness (past 2 weeks)
- •Presence or prior history of cardiac, pulmonary or systemic disease
- •Bleeding disorder, use of systemic anticoagulants or antiplatelet therapy
- •American Society of Anesthesiology (ASA) class 2 or greater
- •Immunocompromised state (HIV, immunoglobulin deficiency, systemic immunosuppressants)
- •Use of any inhaled substance, including tobacco, marijuana, e-cigarrettes, cocaine, methamphetamines, or toxic vapors in the past 3 months or greater than 10 pack-year smoking history
- •Alcohol use disorder or greater than 7 drinks/week for women or greater than 14 drinks/week for men in the past 3 months
- •Allergy or prior adverse reaction to lidocaine, midazolam or fentanyl
- •Abnormal spirometry or electrocardiogram at time of screening
- •Pregnant (based on urine pregnancy test) or breast feeding
Arms & Interventions
LPS arm
Intervention: bronchoscopy with intrabronchial administration of lipopolysaccharide (Other)
Outcomes
Primary Outcomes
Resident alveolar macrophages and recruited macrophages will be distinguished from one another using multi-parameter flow cytometry, and the relative proportions enumerated
Time Frame: 7 days
Resident and recruited macrophages will be purified using fluorescence activated cell sorting (FACS) and subjected to RNA sequencing.
Secondary Outcomes
No secondary outcomes reported