The Effects of Glycogen Availability on the Skeletal Muscle Molecular Response After Concurrent Exercise
Trial Snapshot
- Phase
- Not Applicable
- Status
- Completed
- Sponsor
- Wageningen University
- Enrollment
- 14
- Locations
- 2
- Primary Endpoint
- Proteins involved in hypertrophy
Study Overview
Brief Summary
This is a randomized, counterbalanced crossover design, in which 14 male subjects will complete two experimental trials. Each trial will be separated by a minimum of at least 12 days (range: 12-30 days). The study aims to investigate the effects of low glycogen availability on skeletal muscle molecular response after a concurrent exercise day.
Detailed Description
Two experimental exercise trials; a low carbohydrate (= LOW) and a high carbohydrate (= HIGH) trial. During both experimental days subjects will complete an continues submaximal cycling bout in the morning followed by a resistance exercise session in the early afternoon. During the LOW trial subjects will receive a low carbohydrate mixed meal, during the HIGH trial subjects will be provided with a high carbohydrate meal. In addition, on both experimental days subjects will receive adequate protein beverages. Lastly, protein will be provided post resistance exercise.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Crossover
- Primary Purpose
- Treatment
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to 30 Years (Adult)
- Sex
- Male
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Male gender between 18 and 30 years of age
- •BMI between 18.5 - 25 kg/m2
- •Participating in regular exercise training (at least 1 exercise session, and a maximum of 3 exercise session per week)
- •Used to different types of exercise (endurance, resistance and/or concurrent)
- •Willing to give blood samples
- •Willing to give muscle biopsies
- •No use of antibiotics during the study or in the past month
- •No drug use
- •Suitable veins for blood withdrawal
- •Registered by a general-practitioner
- •Consumption of alcohol beverages is less than 21 per week
- •No blood donor during the study
- •No vegetarian and willing to eat meat during the experimental days
- •Able to be present and participate at both test days and pre testing moments
- •Not employed, or intern, or working on thesis at the department of Human Nutrition at Wageningen University
- •Not participating in another scientific study (except EetMeetWeet)
- •Able to participate during the experimental days en pre tests
- •Able to speak Dutch
Exclusion Criteria
- •Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, gluten intolerance)
- •Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg
- •Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate)
- •Use of antithrombotic therapy (marcoumar, sintromitis).
- •Diagnosed diabetes mellitus type 1 or 2
- •(Chronic) injuries of the locomotor system that can interfere with the intervention
Arms & Interventions
Low carbohydrate
Subjects will eat a low carbohydrate diet
Intervention: Standard meal containing low or high carbohydrate content (Other)
High carbohydrate
Subjects will eat a high carbohydrate diet
Intervention: Standard meal containing low or high carbohydrate content (Other)
Outcomes
Primary Outcomes
Proteins involved in hypertrophy
Time Frame: 10 hours
Secondary Outcomes
No secondary outcomes reported