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Influence of the anesthetic procedure on the exosomal micro-RNA signature in patients undergoing radical cystectomy

Not Applicable
Recruiting
Conditions
C67
Malignant neoplasm of bladder
Registration Number
DRKS00023682
Lead Sponsor
Ruhr-Universität Bochum Marienhospital Herne Klinik für Anästhesiologie, operative Intensivmedizin, Schmerz- und Palliativmedizin
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
All
Target Recruitment
300
Inclusion Criteria

• Written declaration of consent
• planned radical cystectomy with urinary diversion
• Age = 18 years
• Epidural anesthesia in combination with standard anesthesia (balanced or TIVA)
• enhanced recovery after surgery protocol
• ASA II-III

Exclusion Criteria

• Refusal of participation by the patient
• Language barrier
• Age <18 years
• clinically relevant aortic stenosis

Study & Design

Study Type
interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Influence of the anesthetic regimen (epidural anesthesia + balanced anesthesia (sevoflurane) and epidural anesthesia + TIVA propofol / fentanyl) on exosome and miRNA concentration in cystectomy patients<br>Withdrawal of 10 ml of blood from a lying arterial catheter at the following times:<br>1. After arterial catheter placement<br>2. before skin incision<br>3. 30 minutes after cutting<br>4. before skin suture<br>5. after 24 hours.<br>Serum extraction and determination of the exosome concentration in the serum. miRNA extraction from the serum and determination of the miRNA signature in exosomes
Secondary Outcome Measures
NameTimeMethod
Deviations in the micro-RNA signature in the course of anesthesia over time in radical cystectomy<br>comparison of hypotension phases and hemodynamics between groups
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