Evaluation of extraction socket healing with Naturally sourced (Eggshell derived) Nano-hydroxyapatite graft covered with Platelet Rich Fibrin (PRF) and extraction socket filled with Naturally sourced Nano-hydroxyapatite graft alone - A prospective study

This study is a randomized, parallel active controlled trial evaluating the healing of extraction socket with eggshell derived nanohydroxyapatite graft covered with platelet rich fibrin and eggshell derived nanohydroxyapatite graft alone and this study will be conducted in the department of Clinical Research.The study subjects will be enrolled from the out-patient department of Oral andMaxillofacial Surgery. They will be randomly allocated into two groups and theconsent will be obtained from the participants. The study participants willundergo atraumatic extraction. After achievement of post extraction hemostasis,nano-hydroxyapatite graft will be placed into the extraction socket till thecrestal level and PRF membrane will be placed over the grafted socket andtucked between Buccal and Lingual/Palatal gingiva and stabilized using 3-0black silk suture (figure of eight) in one group. In another group,nano-hydroxyapatite graft alone will be placed into extraction sockets up tothe crestal level and will be sutured using 3-0 black silk suture (figure ofeight). After the procedure, the subjects will be advised to follow postextraction instructions. All the subjects will be followed up for 90 days forany adverse events and the parameters like soft tissue healing, pain and alveolar width and height will be assessed on 7th, 14thand 90th day post operatively.

Preparation of Platelet Rich Fibrin (PRF) membrane: This will be prepared using autologous blood under standard asepticprecautions. Intravenous blood of 5 ml will be drawn from the precubital regionusing a sterile disposable syringe. Blood will be centrifuged using a tabletopcentrifuge for 10 minutes at 3000 rpm immediately. The resultant product willconsist of acellular plasma at the top, PRF clot in the middle, and red bloodcells at the bottom. PRF component will be separated and molded into a thinsheet of approximately 1x1 using a sterile wet gauze piece.