Metabolic failure and energy depletion in kidney transplantatio
Completed
- Conditions
- Ischemia/reperfusion injury. Graft failure after transplantation.1003843010038365
- Registration Number
- NL-OMON39126
- Lead Sponsor
- eids Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 30
Inclusion Criteria
Recipients of a kidney graft by kidney tranplantation. (20 recipients)
Living donors (10 donors for a pre-surgical measurement of bloodflow in the a.renalis, non-invasive by echo-doppler).4 of these living donors for 1 arterio and 1 venous sample as control measurement during surgery.
Exclusion Criteria
Increased risk of bleeding.
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Metabolic activity of the kidney as measured by pO2, pCO2, lactate, glucose and<br /><br>full lipid profile. The respiratory quotients (RQ) for every time-point will be<br /><br>calculated. </p><br>
- Secondary Outcome Measures
Name Time Method <p>Kidney biopsies taken before (routine clinical biopsy) and after reperfusion<br /><br>(this study) will be collected in all patients. Biopsy specimens will be snap<br /><br>frozen and processed to make it suitable for NMR. By NMR different<br /><br>intermediates of the citric acid cycle will be quantified, to be able to<br /><br>estimate activity of aerobic metabolism and more accurately to define where<br /><br>potential problems are.<br /><br><br /><br>Since measurements are dependent on flow kinetics and perfusion of the kidney,<br /><br>flow in the renal artery and microvascular perfusion will be measured as well.<br /><br>Both will be compared between living and cadaveric donor kidneys. Measurements<br /><br>of metabolism will be related to microvascular perfusion<br /><br>We will also measure renal blood flow in the living donor and compare this to<br /><br>the renal blood flow through the same kidney after transplantation.</p><br>