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Lucky Iron Fish Home Fortification of Iron

Not Applicable
Completed
Conditions
Anemia, Iron-Deficiency
Anemia
Hemoglobinopathies
Interventions
Other: Nutrition Education
Other: Lucky Iron Fish - home fortification system
Dietary Supplement: 18 mg elemental iron
Registration Number
NCT02341586
Lead Sponsor
University of British Columbia
Brief Summary

The purpose of this research is to determine if cooking with an iron ingot called the Lucky Iron Fish (LIF) increases the hemoglobin status in women of childbearing age living in Preah Vihear, Cambodia. The investigators hypothesize that the use of the LIF during cooking over a 12-month period will be as efficacious at increasing hemoglobin concentration as iron supplements (18 mg elemental iron) and will be more efficacious than the control.

Detailed Description

Background: Anemia is prevalent in Cambodia, affecting \~44% of women. The causes of anemia are multifactorial. Globally, the most common form is iron deficiency anemia (IDA), which can lead to adverse perinatal health outcomes. In Cambodia, there is evidence that suggests hemoglobinopathies (genetic hemoglobin \[Hb\] disorders) are prevalent (\~50%) and are a major contributor to anemia. In Preah Vihear province, Cambodia, the estimated prevalence of malaria is \~12.3%. Malaria contributes to anemia through a decrease in the production of red blood cells, and an increased destruction of red blood cells.

In Cambodia, iron supplements are recommended to treat anemia, however there is evidence of low compliance among women. The use of cast iron pots has proven to be an effective alternative to prevent and reduce IDA in developing countries. However, cast iron pots are not commonly used in Cambodia because they are expensive, difficult to clean, and heavy. A novel alternative is the Lucky Iron Fish (LIF), which is an iron ingot used during cooking as an in-home fortification system of iron transfer. The LIF ingot is small, lightweight, and easy to clean. Previous research in Cambodia confirmed the acceptance of the LIF to be high, but findings from this study were inconclusive and had several limitations.

Objective: The primary objective of this study is to determine the efficacy of the LIF to increase Hb concentration in Cambodian women of reproductive age.

Methods: A total of 330 women (18-49 y) with mild or moderate anemia (Hb 80-120 g/L) from rural Preah Vihear, Cambodia will be recruited to one of three arms to receive LIF, 18 mg elemental iron, or a placebo. Women with severe anemia will be excluded and referred for treatment. All three groups will receive nutrition education. Monitoring will be conducted monthly to measure compliance.

A trained, Khmer-speaking interviewer will meet with the women to explain the details of the study as per the consent form. Once consent is obtained the trained interviewer will measure hemoglobin status using a hemocue device to confirm eligibility of the study. If eligible, the trained interviewer will then administer the baseline questionnaire in the participant's home.

The questionnaire will collect demographic data, as well as information on current dietary intake, drinking water treatment, knowledge of iron deficiency anemia, knowledge of malaria, and perceptions of supplement use.

Participants will have a venous blood sample taken at 0, 6, and 12 months and analyzed for hemoglobin and multiple biomarkers of iron status (e.g. soluble transferrin receptor, ferritin, and retinol binding protein (RBP)). Ferritin will be corrected for inflammation using measures of alpha-1 acid glycoprotein (AGP, g/L) and C-reactive protein (CRP, mg/L) which are biomarkers of chronic and acute inflammation, respectively.

Genotyping will be conducted to determine the prevalence of hemoglobinopathies among women. The prevalence of malaria in this cohort will be determined using rapid diagnostic test kits for P. Falciparum, and P. Vivax at baseline, midline, and endline.

Potential Significance: The LIF has the potential to be a low cost, effective and simple-to-use product to prevent and reduce iron deficiency anemia in Cambodia.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
340
Inclusion Criteria
  • Mild or Moderate Anemia [Hb 80-119]
  • Willing to provide a fingerprick sample of blood at baseline
  • Willing to provide 10 mL of venous blood at 0, 6 & 12 months
  • Not planning on moving in the next 12 months
  • Not participating in any other nutrition intervention
  • Be able to provide written and information consent
Read More
Exclusion Criteria
  • Be ill or taking any medications [Hb < 80]
  • Have normal Hb status [Hb > 120]
  • Currently consuming or planning to consume Fe supplements in the next 12 months
  • Pregnant (based on self report)
Read More

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Control groupNutrition EducationThis group will receive nutrition education
Lucky Iron FishLucky Iron Fish - home fortification systemThis group will receive a Lucky Iron Fish to use during cooking.
18 mg iron18 mg elemental ironThis group will receive a daily oral iron supplement.
Primary Outcome Measures
NameTimeMethod
hemoglobin concentration at endline12 months
Secondary Outcome Measures
NameTimeMethod
Change in soluble transferrin receptor (sTfR) concentrationBaseline (t=0), midline (t=6 months), and endline (t=12 months)

sTfR is a biomarker of iron status

How the prevalence of malaria changes in women living in Preah Vihear Cambodia over one yearBaseline (t=0), midline (t=6 months), and endline (t=12 months)
Change in ferritin concentrationBaseline (t=0), midline (t=6 months), and endline (t=12 months)

Ferritin is a biomarker of iron status. Ferritin will be corrected with measures using measures of alpha-1 acid glycoprotein and C-reactive protein.

Number of women in Preah Vihear, Cambodia with a genetic hemoglobin disorderMidline (t=6 months)

Trial Locations

Locations (1)

Rovieng Health Centre

🇰🇭

Rovieng, Preah Vihear, Cambodia

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