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Correlative Study on Cancer Patients and Healthcare Professionals Exposed to Infection by Severe Acute Respiratory Syndrome-Corona Virus-2 (SARS-Cov-2), COVID-19 Causative Agent.

Conditions
SARS-CoV-2
COVID-19
Interventions
Genetic: Next generation Sequencing (NGS) analysis
Other: serological test
Other: Rapid molecular test
Other: serum chemistry analysis
Registration Number
NCT04345315
Lead Sponsor
Istituto Scientifico Romagnolo per lo Studio e la cura dei Tumori
Brief Summary

Translational, prospective / retrospective, non-profit, non-pharmacological study, with cohort characteristics.

The study consists of two parts: the first to study epidemiological aspects of the spread of the disease and the second one to identify infection-related genetic factors.

Detailed Description

The outbreak of COVID-19 infections is spreading in Italy with unprecedented severity characteristics. In this context, it is essential to collect data relating to the epidemiology of the disease, to outline further useful tools for diagnosis and to define the correct use of rapid molecular and / or serological tests in the surveillance of high-risk subjects (cancer patients and healthcare operators), in order to identify any new disease control strategies, crucial to reduce the transmission and to outline specific guidelines for cancer patients healthcare management. It is also necessary to identify the factors that determine the evolution of the viral genome over time in the various geographical areas and also the potential susceptibility markers in the different types of affected subjects (symptomatic, mildly symptomatic and asymptomatic).

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
500
Inclusion Criteria
  • Age > 18 years
  • asymptomatic
  • One of the following three conditions: • Healthy subjects who have had contact with COVID-19 positive patients • Healthcare workers • oncological patients candidates for cytotoxic chemotherapy at myeloablative doses (such as transplants) or treated with immune-check point inhibitors.
  • informed consent to the study and processing of data
Exclusion Criteria
  • presence of symptoms for COronaVIrus Disease 19 (COVID-19) (fever > 37.5 °, cough, shortness of breath)

For analysis of viral/host genetic factors involved in susceptibility/resistance to infection, samples from patients with confirmed diagnosis of COVID-19, residual from diagnostic procedures will be analyzed. All samples for genetic analysis will be anonymized.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
asymptomatic population at high risk of infectionserum chemistry analysishealthy individuals at high risk of infection, including individuals who have had contact with a patient tested positive for COVID-19, voluntary health workers and oncological patients candidate to immunosuppressive therapy
COVID-19 patientsNext generation Sequencing (NGS) analysispatients with confirmed diagnosis of COVID-19
asymptomatic population at high risk of infectionserological testhealthy individuals at high risk of infection, including individuals who have had contact with a patient tested positive for COVID-19, voluntary health workers and oncological patients candidate to immunosuppressive therapy
asymptomatic population at high risk of infectionRapid molecular testhealthy individuals at high risk of infection, including individuals who have had contact with a patient tested positive for COVID-19, voluntary health workers and oncological patients candidate to immunosuppressive therapy
Primary Outcome Measures
NameTimeMethod
epidemiology12 months

Investigate the epidemiology of the infection in an asymptomatic population including both healthy individuals at high risk of infection and oncological patients by assessing the seroprevalence of IgG and IgM antibodies against the SARS-CoV-2

Secondary Outcome Measures
NameTimeMethod
methods comparison24 months

Make a comparison between different serological investigation methods and rapid molecular methods becoming available

Immunoglobulin G (IgG) and Immunoglobulin M (IgM) antibodies evaluation12 months

IgG and IgM antibodies evaluation over time

phylogenetic map24 months

Building a phylogenetic map of an epidemic Italian macro-region

correlation between biochemical and coagulative factors with SARS-CoV-2 positivity.24 months

To evaluate correlation between biochemical and coagulative factors with SARS-CoV-2 positivity

interactions between the virus and host cells24 months

Evaluate the spectrum of possible interactions between the virus and host cells, considering their genetic variability / instability in patients diagnosed with COVID-19

Trial Locations

Locations (2)

Irst Irccs

🇮🇹

Meldola, FC, Italy

UO Microbiologia,Centro Servizi Pievesestina, AUSL Romagna

🇮🇹

Cesena, FC, Italy

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