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Modulation of Insulin Sensitivity by Betaine Upregulation of FGF21

Not Applicable
Completed
Conditions
Insulin Sensitivity
Interventions
Dietary Supplement: Dextrose
Dietary Supplement: Betaine
Registration Number
NCT02118142
Lead Sponsor
UNC Nutrition Research Institute
Brief Summary

People with poor insulin sensitivity do not respond normally to elevations in blood sugar. This may increase their risk of developing diabetes in the future. The purpose of this research study is to determine if the nutrient betaine, found in beets, spinach and wheat products, can enhance the production of fetal growth factor 21 (FGF21), a molecule that is believed to promote insulin sensitivity.

Detailed Description

AIM: To determine if betaine is a modulator of FGF21 in humans. Low plasma betaine is associated with an increased risk of metabolic syndrome, but the mechanisms modulating this correlation are poorly delineated. Betaine bioavailability in humans is determined by dietary intake and genetic polymorphisms that influence betaine metabolism. Therefore, moderating betaine levels could be particularly beneficial for some individuals. FGF21 is elevated in response to insulin insensitivity and is under active investigation as a therapeutic modality. The mechanisms of action of FGF21 and betaine on insulin sensitivity in humans are incompletely understood. This study tests the hypothesis that betaine induces FGF21 secretion and insulin sensitizing actions in humans by measuring plasma FGF21, betaine, choline (betaine precursor), glucose, insulin and adiponectin in response to betaine supplementation over a 24 hour time course in 20 healthy, lean individuals. This pilot nutrition intervention will provide key preliminary evidence for understanding whether and how betaine exerts metabolic benefit in humans and will inform a future study to investigate the novel betaine-FGF21-insulin sensitivity axis in a larger cohort.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
23
Inclusion Criteria
  • Healthy
  • Able/willing to consume study meals
  • Non-smoker
  • BMI in normal range (18-24.9)
Exclusion Criteria
  • Use of chronic medications
  • Abnormal physical examination or chronic illness
  • Use of drugs or medications known to alter choline/betaine metabolism
  • Consumption of more than 2 oz of alcohol/day or 24 oz wine/day
  • Use of choline/betaine-containing dietary supplements during the previous 3 months
  • Diagnosed with Cystathionine Beta-Synthase (CBS) Deficiency
  • Pregnant or breastfeeding
  • Known hypersensitivity to betaine
  • Current substance abuse or addiction

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
DextroseDextrose6 gram dose of dextrose delivered in encapsulated form
BetaineBetaine6 gram dose of betaine delivered in encapsulated form
Primary Outcome Measures
NameTimeMethod
Plasma glucose concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Plasma Fetal Growth Factor 21 (FGF21) concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Plasma choline concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Plasma betaine concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Plasma insulin concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Plasma adiponectin concentration0, 0.5, 1, 2, 4, 8, and 24 hours post- betaine or placebo supplement
Secondary Outcome Measures
NameTimeMethod
Typical dietary intakeDuring day 2 of the first 3 day phase

Assessed via questionnaire

Typical exercise habitsDuring day 2 of the first 3 day phase

Assessed via questionnaire

Trial Locations

Locations (1)

UNC Nutrition Research Institute

🇺🇸

Kannapolis, North Carolina, United States

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