1. Study Design 2. Sample Collection 3. Microbial DNA Extraction in Faecal Samples 4. PCR Amplification： 5. Sequencing： 6. HCY Test Principle: 7. Statistical Analysis
概览
- 阶段
- 不适用
- 干预措施
- 未指定
- 疾病 / 适应症
- Recurrent Miscarriage
- 发起方
- First People's Hospital of Hangzhou
- 入组人数
- 38
- 试验地点
- 1
- 主要终点
- homocysteine levels
- 状态
- 已完成
- 最后更新
- 2年前
概览
简要总结
The goal of this observational study is to test the alterations of gut microbiota composition among women of reproductive age with elevated homocysteine levels. The main question it aims to answer is:
• the relationship between gut microbiota composition and recurrent abortion. Participants will provide their stool samples to be detected the composition of gut microbiota. .
Researchers will compare women of reproductive age with normal homocysteine levels to see if any bacteria were involved in recurrent miscarriages.
研究者
入排标准
入选标准
- •No recent antibiotic treatment
排除标准
- •Recently received antibiotics
结局指标
主要结局
homocysteine levels
时间窗: The first day of the trail
Binding or dimer homocysteine (oxidized form) is reduced to free homocysteine, and then it is catalysed by cysteine β-synthetase (CBS) to react with serine to form cysteine sulfuric acid. Cysteine is then broken down by cysteine β-lyase (CBL) to form homocysteine, pyruvate, and ammonia. Pyruvate is then converted to lactate using lactate dehydrogenase (LDH), where nicotinamide adenine dinucleotide (NADH) is used as a coenzyme. The ratio of NADH to NAD\* is directly proportional to the concentration of homocysteine (△ A340 nm).