Effect of Baseline Ferritin Level on Iron Status in Male Elite Youth Rowers
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 16
- 试验地点
- 1
- 主要终点
- The Blood morphology- MCHC (Mean Corpuscular Hemoglobin Concentration)
研究概览
简要总结
The data of 16 trained men from the Polish Youth National Rowing. All athletes presented as medically fit, with no underlying health problems. In this cohort, baseline serum ferritin levels were considered, and the athletes were divided into two groups:
- Serum ferritin <75 ng/ml (SF<75): n=9
- Serum ferritin >75 ng/ml (SF>75): n=7
The rowing test was performed at the beginning of the preparatory phase of the annual training cycle (April), which is characterized by high volume and training load. After a 5-min of individual warm-up, the athletes performed a 2000-m exercise test on a rowing ergometer (Concept II, USA). Blood samples took from the cubital vein at three time points: before stress test (pre-exercise), one minute after the end of the test (post-exercise), and after a 1-hour recovery period (1h recovery).
Measurements ferritin, transferrin, hepcidin and interleukin-6 (IL-6) levels were determined using ELISA kits (SunRed Biotechnology Company). The iron levels were determined on the SPECTROstar Nano reader.
详细描述
The data of 16 trained men from the Polish Youth National Rowing. All athletes presented as medically fit, with no underlying health problems. In this cohort, baseline serum ferritin levels were considered, and the athletes were divided into two groups:
- Serum ferritin <75 ng/ml (SF<75): n=9
- Serum ferritin >75 ng/ml (SF>75): n=7
The rowing test was performed at the beginning of the preparatory phase of the annual training cycle (April), which is characterized by high volume and training load. After a 5-min of individual warm-up, the athletes performed a 2000-m exercise test on a rowing ergometer (Concept II, USA). Blood samples took from the cubital vein at three time points: before stress test (pre-exercise), one minute after the end of the test (post-exercise), and after a 1-hour recovery period (1h recovery).
Measurement: All determined parameters will be measured with the available equipment in the ZWKF laboratory in Gorzów Wielkopolski and using commercial assay kits. Measurements will be performed by the project contractors.
- Polyethylene tubes (2.7ml) containing dipotassium ethylenediaminetetraacetic acid (EDTAK2) anticoagulant will be used for the following tests:
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Retrospective
入排标准
- 年龄范围
- 18 Years 至 24 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 否
入选标准
- •Training experience a minimum of five years
- •Minimum training time per week 240 minute
- •Membership in the Youth National Rowing Team of Poland
- •Completion of the 2000m ergometer test on two test dates
排除标准
- •Taking other supplements containing anthocyanin compounds during the study period or a month earlier
- •Antibiotic therapy
- •Health problems
结局指标
主要结局
The Blood morphology- MCHC (Mean Corpuscular Hemoglobin Concentration)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
MCHC (Mean Corpuscular Hemoglobin Concentration) \[g x dL-1\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
The Blood morphology- RDW (Red Blood cells Distribution Width)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
RDW (Red Blood cells Distribution Width) \[%\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
Ferritin
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Concentration of ferritin \[ng/mL\]. ELISA method by the test manufacturer's instructions
Iron
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
The iron level \[µg/dL\] will be determined using the colorimetric method on the SPECTROstar Nano reader.
Interleukin-6 (Il-6)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Concentration of IL-6 \[ng/L\]. ELISA method by the test manufacturer's instructions
Hepcidin
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Concentration of hepcidin \[ng/mL\]. ELISA method by the test manufacturer's instructions
Transferrin
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
Concentration of transferrin \[mg/mL\]. ELISA method by the test manufacturer's instructions
The Blood morphology - RBC (Red Blood Cells)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
RBC (Red Blood Cells) \[106 × µL-1\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
The Blood morphology- HGB (Hemoglobin)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
HGB (Hemoglobin) \[g/dL\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
The Blood morphology- HCT (Hematocrit)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
HCT (Hematocrit) \[%\]determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
The Blood morphology- MCV (Mean Corpuscular Volume)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
MCV (Mean Corpuscular Volume)\[fl\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
The Blood morphology- MCH (Mean Corpuscular Hemoglobin)
时间窗: At rest (before the test), directly after the test, and after a 1-hour recovery period.
MCH (Mean Corpuscular Hemoglobin) \[pg\] determined on the MYTHIC 18 hematology analyzer (Orphee Medical, Geneva, Switzerland).
次要结局
- Antropometric characteristic - height(Day 1 after overall fast)
- Antropometric characteristic - weight(Day 1 after overall fast)
- Food record - energy(Day before the Day 1, in the morning at Day 1)
- Food record - protein(Day before the Day 1, in the morning at Day 1)
- Food record - carobhydrates(Day before the Day 1, in the morning at Day 1)
- Food record - fiber(Day before the Day 1, in the morning at Day 1)
- Food record - fat(Day before the Day 1, in the morning at Day 1)