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Effect of Polyphenol Supplementation on Hepatic Steatosis and Vascular Compliance

Not Applicable
Recruiting
Conditions
Hepatic Steatosis
Intimal Hyperplasia
Obesity, Childhood
Interventions
Dietary Supplement: Effect of polyphenols on hepatic steatosis and vascular elastography and intima media thickness
Registration Number
NCT03994029
Lead Sponsor
St. Justine's Hospital
Brief Summary

The main objective is to study the effect of polyphenol supplementation on hepatic steatosis as measured by hepatic ultrasound, hepatic magnetic resonance imaging and on intima-media thickness and vascular elastography in obese adolescents known for hepatic steatosis as diagnosed by liver biopsy

Detailed Description

1. Evaluate the feasibility of a larger, open-label, randomized, controlled trial on the effects of polyphenol supplementation:

Recruitment, compliance to polyphenol supplementation and the visit roadmaps Duration of the radiological exams and participants' satisfaction and point of view on the experience. Test the relevance of using a food diary. Test the effectiveness of the data collection procedure during the visits. Explore the obstacles encountered while performing the radiological exams and the rate of adverse events (AE). Calculate the sample size of the future randomized clinical study

2. Effects of polyphenols IMT and vascular elastography. Anthropometric measurements, insulin resistance, inflammation, lipid/lipoprotein profile, gut microbiome and liver function

3. Evaluated different imaging modality for steatosis Different imaging modalities will be compared between children

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
60
Inclusion Criteria
  • Aged 12 to 18 years
  • BMI percentile > 85th for age and sex
  • Diagnosis of hepatic steatosis on imaging (ultrasound or MR).
  • Diagnosis of hepatic steatosis, NASH, or fibrosis on liver biopsy.
  • Elevated alanine aminotransferase (ALT) enzyme level.
  • Index of hepatic steatosis 8xALT/AST + BMI (+2 for girls) > 30.
Exclusion Criteria
  • Known chronic systematic diseases
  • Any other serious conditions which, according to the doctor's judgment, would prevent compliance and safe participation in the study until completion.

Exclusion criteria to be validated when contacting the participants and their parents/tutors:

  • Being pregnant.
  • Taking all kinds of prescription or over-the-counter natural health products/natural supplements/vitamins on an ongoing basis or within the next four months, excluding vitamin D.
  • Weight loss of 5% to 10% of the usual weight in the last six months before recruitment or weight change of 5% in the last three months.
  • Alcohol consumption > two drinks/day or > one day/week.
  • Known peanut allergy and/or to the medicinal ingredients contained in the active polyphenol supplement: elderberry, haskap, black chokeberry, blueberry, blackcurrant.
  • Any contraindications for MRI.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Polyphenol supplementationEffect of polyphenols on hepatic steatosis and vascular elastography and intima media thickness120mg per day of powder polyphenol for 60 days
PlaceboEffect of polyphenols on hepatic steatosis and vascular elastography and intima media thickness1 tab PO QD per day of placebo for 60 days
Primary Outcome Measures
NameTimeMethod
RecruitmentThrough study completion an average of 1 year

Rate of recruitment

Satisfaction of the participants and their point of view on the experienceThrough study completion an average of 1 year

Percentage of satisfaction of participants enrolled

Retention:Through study completion an average of 1 year

The percentage of adolescents who agree to participate in the study and the retention

Compliance with polyphenol supplementationThrough study completion an average of 1 year

Percentage of compliance

Adverse eventThrough study completion an average of 1 year

Percentage of adverse events

Effectiveness of the data collection procedure during the visitsThrough study completion an average of 1 year

Percentage of completed visits

Sample size calculation for a randomized controlled studyThrough study completion an average of 1 year

A sample size for a future randomized controlled study will be calculated from the estimates obtained during this pilot study.

Secondary Outcome Measures
NameTimeMethod
Change in hepatic steatosis MRAt recruitment, after 60 days, and after 120 days

mDixonQuant in percentage

Stool testAt recruitment, after 60 days, and after 120 days

Gut microbiome composition

Change in hepatic steatosis B-mode USAt recruitment, after 60 days, and after 120 days

US liver echogenicity normal or increased

Inflammatory marker 2At recruitment, after 60 days, and after 120 days

TNF alpha

Lipid markers 1At recruitment, after 60 days, and after 120 days

Triglycerides

Lipid markers 3At recruitment, after 60 days, and after 120 days

HDL

CholesterolAt recruitment, after 60 days, and after 120 days

Triglyceride index (no unit)

Anthropometric measureAt recruitment, after 60 days, and after 120 days

Body mass index

Lipid markers 6At recruitment, after 60 days, and after 120 days

Adiponectin, leptin

Change in intima media thickness (vascular compliance)At recruitment, after 60 days, and after 120 days

Measurement of carotid artery intima media thickness in mm

Metabolic syndromeAt recruitment, after 60 days, and after 120 days

Insulin resistance evaluated by HOMA-IR mg\\dl

Inflammatory marker 3At recruitment, after 60 days, and after 120 days

IL-6

Lipid markers 4At recruitment, after 60 days, and after 120 days

Apo AI

Liver function testAt recruitment, after 60 days, and after 120 days

ALT

Inflammatory marker 1At recruitment, after 60 days, and after 120 days

CRP

Lipid markers 2At recruitment, after 60 days, and after 120 days

LDL

Lipid markers 5At recruitment, after 60 days, and after 120 days

Apo B-100

Trial Locations

Locations (1)

CHU Sainte-Justine

🇨🇦

Montreal, Quebec, Canada

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