Comparative analysis of the effect of calorie free sweeteners and probiotics on the oral microbial concentration in children: a randomised controlled trial

This randomized controlled trial compares the effects of calorie-free sweeteners (aspartame, stevia, xylitol) and probiotics on oral microbial concentration in children aged 6-12. A total of 120 participants are randomly assigned into four groups of 30. Group 1 receives aspartame, Group 2 receives stevia, Group 3 receives xylitol, and Group 4 receives probiotics. The intervention lasts for 4 weeks, with follow-up assessments at baseline, 1 week, and 4 weeks. Saliva samples are collected at baseline, 1 hour after consumption, and at subsequent time points to measure oral microbial concentration using quantitative PCR (qPCR) and microbial culture techniques.

Secondary outcomes include changes in gingival health and caries status, evaluated through the gingival index, Oral Hygiene Index (OHI), and International Caries Detection and Assessment System (ICDAS) index. Children with systemic diseases, recent dental treatments, or allergies to probiotics/sweeteners are excluded. Compliance is monitored by parents using a "sweet diary."

The study provides insight into the potential effects of calorie-free sweeteners and probiotics on children’s oral microbial health and dental outcomes, including caries risk and gingival health.