Quantification of Ketosis After Intake of Coconut Oil and Caprylic Acid-With and Without Glucose-After a 12-hour Fast: A 6-way Cross-over Study in Healthy Older Adults
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 15
- 试验地点
- 2
- 主要终点
- Concentrations of BHB, area under the curve, 0-4 h
研究概览
简要总结
In a cross-over design, 15 participants, age 65-75, will be tested in six different arms in a randomised order. After a 12-hour fast participants will be served 2.5 dl coffee with 15 g cream + 30-50 g of different fatty acids, with or without 50 g glucose. Blood ketones and other biomarkers will be measured during 4 hours.
详细描述
In the metabolic state ketosis, beta-hydroxybutyrate (BHB) and acetoacetate are synthesised from fatty acids in the liver, resulting in higher circulating levels. Ketosis can be induced by fasting or adherence to a low-carb-high-fat diet, but also by intake of-at least some-medium-chain-triglycerides (MCT). MCT are built from fatty acids with a chain of 6-12 carbon atoms. Caprylic acid (C8) is well known to be ketogenic, but the effect of lauric acid (C12)-constituting about 50% of coconut oil-is less clear.
The aim of this study is to assess ketosis, as a combined effect of an extended overnight fast, and intake of coconut oil or C8, and to study how ketosis is affected by simultaneous glucose intake. Sunflower oil is used as control, expected to not break fasting ketosis, although not being ketogenic in itself. Some sunflower oil is also added to C8 to make that arm isocaloric with coconut oil, with a roughly similar proportion of MCT.
Coffee with cream will be used as a vehicle, and although coffee has been reported to be mildly ketogenic, this effect is expected to be negligible and equally distributed between arms.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- Single (Participant)
入排标准
- 年龄范围
- 65 Years 至 75 Years（Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Daily consumption of coffee
- •Written informed consent
排除标准
- •Weight <50 kg
- •Diagnosed diabetes
- •History of heart disease
- •History of disease related to internal organs or metabolism
- •Experience of "sensitive gut", or known intolerance to the test ingredients
- •Medication expected to affect glucose- or lipid-metabolism
- •Fasting during study or one month before
- •High intensity physical activity > 3 times/week
- •Severe psychiatric conditions
- •Hb<125 g/L
- •Participation in other lifestyle intervention last 6 months
研究组 & 干预措施
3. Caprylic acid + Glucose
Caprylic acid (20 g) + Sunflower oil (10 g) + Glucose (50 g)
干预措施: Caprylic acid (Dietary Supplement)
3. Caprylic acid + Glucose
Caprylic acid (20 g) + Sunflower oil (10 g) + Glucose (50 g)
干预措施: Sunflower oil (Other)
4. Coconut oil
Coconut oil (30 g)
干预措施: Coconut oil (Other)
1. Sunflower oil
Sunflower oil (30 g)
干预措施: Sunflower oil (Other)
2. Caprylic acid
Caprylic acid (20 g) + Sunflower oil (10 g)
干预措施: Caprylic acid (Dietary Supplement)
5. Coconut oil + Glucose
Coconut oil (30 g) + Glucose (50 g)
干预措施: Coconut oil (Other)
5. Coconut oil + Glucose
Coconut oil (30 g) + Glucose (50 g)
干预措施: Glucose (Other)
3. Caprylic acid + Glucose
Caprylic acid (20 g) + Sunflower oil (10 g) + Glucose (50 g)
干预措施: Glucose (Other)
6. Coconut oil + Caprylic acid
Coconut oil (30 g) + Caprylic acid (20 g)
干预措施: Coconut oil (Other)
2. Caprylic acid
Caprylic acid (20 g) + Sunflower oil (10 g)
干预措施: Sunflower oil (Other)
6. Coconut oil + Caprylic acid
Coconut oil (30 g) + Caprylic acid (20 g)
干预措施: Caprylic acid (Dietary Supplement)
结局指标
主要结局
Concentrations of BHB, area under the curve, 0-4 h
时间窗: 0, 15, 30, 45, 60, 75, 90, 120, 150, 180, 210, 240 (minutes)
BHB, measured in venous whole blood with a point-of-care meter
次要结局
- Concentrations of total ketones(0, 30, 60, 120 (minutes))
- Concentrations of BHB (capillary)(30, 60, 120 (minutes))
- Concentrations of BDNF / pro-BDNF(0, 60, 120, 240 (minutes))
- Self-rated tolerance of beverage(240 (minutes))
- Concentrations of glucose(0, 60, 120, 180, 240 (minutes))
- Self-rated satiety(240 (minutes))
研究者
Miia Kivipelto
Professor, MD, PhD
Karolinska Institutet