The Role of CSF in Chiari II Brain Malformation
- Conditions
- MyelomeningoceleBrain MalformationChiari Malformation Type 2
- Interventions
- Other: collection of cerebrospinal fluid
- Registration Number
- NCT06560788
- Lead Sponsor
- University College, London
- Brief Summary
Spina bifida, particularly its most severe form known as open spina bifida (myelomeningocele), is a significant congenital disorder that results in profound neurological impairments, including Chiari II malformation. This malformation is associated with the downward displacement of the cerebellum and brainstem into the spinal canal, often leading to hydrocephalus, a condition where cerebrospinal fluid (CSF) accumulates in the brain1. These conditions can result in a range of complications, including cognitive and motor disabilities, learning difficulties, and, in severe cases, early mortality1,2.
While surgical interventions, including prenatal and postnatal surgeries, have been developed to manage the physical manifestations of spina bifida and Chiari II malformation, these procedures have not been fully successful in addressing the associated brain anomalies3. This study aims to explore the hypothesis that the composition of CSF plays a critical role in the development of these brain defects. Specifically, it is hypothesized that the rapid replenishment of CSF, due to its leakage from the open spine in spina bifida, results in a "less mature" fluid composition, which negatively affects neurogenesis and neuronal migration during critical periods of brain development.
- Detailed Description
Study Population and Methodology
This prospective case-control study will involve the collection of CSF samples from several groups, including:
1. Newborns with open spina bifida undergoing postnatal surgery.
2. Fetuses undergoing prenatal surgery for spina bifida.
3. Newborns with hydrocephalus undergoing shunt surgery (control group).
4. Infants undergoing spinal surgery for conditions unrelated to spina bifida (control group).
5. Age-matched fetuses obtained from the Human Developmental Biology Resource (HDBR) as controls.
6. Mouse models: This includes a genetic mouse model of spina bifida (Cdx2Cre x Pax3flox) and normal (wild-type) mice as controls
These samples will be analyzed using mass spectrometry-based proteomics to identify differences in protein composition and concentrations between the groups. Additionally, brain slices from human embryos and mouse models will be cultured in the presence of these CSF samples to assess the impact on neurogenesis and neuronal migration.
Expected Benefits The findings from this study are expected to provide new insights into the pathogenesis of Chiari II malformation and other associated brain anomalies in children with spina bifida. By understanding how CSF composition influences brain development, the study could pave the way for novel therapeutic strategies aimed at modifying CSF composition during early pregnancy. This could potentially prevent or mitigate the neurological impairments associated with spina bifida, ultimately improving the quality of life for affected individuals.
Impact on Clinical Practice and Policy Should the study confirm the hypothesis, it could lead to changes in clinical practices concerning the management of spina bifida and Chiari II malformation. For instance, it might inform the development of new prenatal treatments or interventions designed to normalize CSF composition before significant brain damage occurs4-6. This would represent a significant advancement in fetal surgery and pediatric neurosurgery, with the potential to influence guidelines and policies within the NHS and other healthcare systems globally.
Relation to Academic Qualification This study is being conducted as part of the Lewis Spitz PhD program at University College London (UCL) and Great Ormond Street Institute of Child Health (GOSH ICH). The research builds upon previous studies sponsored by UCL-ICH/GOSH, particularly those investigating the neurodevelopmental consequences of spina bifida and related congenital conditions.
Recruitment & Eligibility
- Status
- NOT_YET_RECRUITING
- Sex
- All
- Target Recruitment
- 18
Newborns with Spina Bifida (Postnatal Closure)
- Diagnosed with open spina bifida (myelomeningocele).
- Scheduled for postnatal surgical closure of the spinal lesion at Great Ormond Street Hospital (GOSH).
- Age: Between 1 day to 1 year old.
Control Group 1 (Newborns with Hydrocephalus)
- Newborns scheduled for shunt surgery for hydrocephalus unrelated to spina bifida.
- Age and sex matched to the spina bifida newborns as closely as possible.
- Age: Between 1 day to 1 year old.
Control Group 2 (Infants with Spinal Conditions Unrelated to Spina Bifida)
- Infants undergoing paned spinal surgery for conditions such as spinal lipoma, fatty filum, tethered cord, etc.
- Age and sex matched to the spina bifida newborns as closely as possible.
- Age: Between 1 day to 1 year old. Fetuses with Spina Bifida (Prenatal Closure)
- Prenatal diagnosis of spina bifida (myelomeningocele) and scheduled for fetal surgery at UCLH.
- Reviewed by Mr Thompson at his outpatient clinic at GOSH
- Gestational age: Between 22 and 24 weeks.
Control Fetal Samples
- Aborted fetuses within the gestational age range of 22-24 weeks.
- Samples obtained through the Human Developmental Biology Resource (HDBR).
Mouse Models
- Genetic mouse model of spina bifida (Cdx2Cre x Pax3flox).
- At embryonic day (E)13.5 (end of the embryonic period) and E18.5 (just before birth)
Control Mouse Models
- Normal (wild-type) mice to serve as controls.
- Normal brain development
- At embryonic day (E)13.5 (end of the embryonic period) and E18.5 (just before birth)
Newborns with Spina Bifida (Postnatal Closure)
- Newborns who have undergone previous surgical intervention.
- Presence of additional unrelated congenital anomalies that could affect cerebrospinal fluid (CSF) composition like meningitis or intraventricular bleeding
- Older than 1 year and 1 month of age.
- Parents refused to participate
- Native language different to English with no translation services available
Control Group 1 (Newborns with Hydrocephalus)
- Newborns with hydrocephalus caused by spina bifida.
- Presence of intraventricular infection or haemorrhage.
- Older than 1 year and 1 month of age.
- Parents refused to participate
- Native language different to English with no translation services available
Control Group 2 (Infants with Spinal Conditions Unrelated to Spina Bifida)
- Infants who were born with spina bifida
- Infants with coexisting conditions that could affect CSF composition like intraspinal tumours, empyema or haemorrhage.
- Older than 1 year and 1 month of age.
- Parents refused to participate
- Native language different to English with no translation services available
Fetuses with Spina Bifida (Prenatal Closure)
- Fetuses with additional major anomalies unrelated to spina bifida like diaphragmatic hernia.
- Gestational age outside the range of 22-24 weeks.
- Surgery performed by other neurosurgery team (not GOSH)
- Parents refused to participate
- Native language different to English with no translation services available
Control Fetal Samples
- Poorly preserved aborted fetuses not suitable for CSF collection.
- Gestational age outside the range 22-24 weeks.
Mouse Models
- Mice with any genetic modifications other than those specified for the spina bifida model.
- Mice with other congenital or acquired anomalies affecting the central nervous system.
Control Mouse Models
● Mice with any genetic modifications or health conditions that could influence the study's outcomes.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Control group 1: Newborns with hydrocephalus undergoing shunt surgery, unrelated to spina bifida collection of cerebrospinal fluid CSF will be collected during suregry at GOSH Case group 1: Children with Open Spina Bifida and Chiari II Malformation collection of cerebrospinal fluid CSF will be collected during postnatal surgical repair at Great Ormond Street Hospital (GOSH) Case group 2: Fetuses Undergoing Prenatal Surgery for Spina Bifida collection of cerebrospinal fluid CSF will be collected during prenatal surgery at University College London Hospitals (UCLH). Control group 2: Aborted fetuses within the gestational age range of 22-24 weeks, collection of cerebrospinal fluid CSF will be collected from fetuses provided by the Human Developmental Biology Resource (HDBR). Case group 3: mouse model of spina bifida (Cdx2Cre x Pax3flox) collection of cerebrospinal fluid CSF will be collected Control group 1bis: Infants undergoing spinal surgery for conditions other than SB collection of cerebrospinal fluid CSF will be collected during surgery at GOSH. Control group 3: normal (wild-type) mice collection of cerebrospinal fluid CSF will be collected
- Primary Outcome Measures
Name Time Method Identification of Proteins in Cerebrospinal Fluid (CSF) 1 year The presence of specific proteins in cerebrospinal fluid (CSF) will be identified through proteomic analysis. This outcome will focus on categorizing which proteins are present in the CSF samples from patients with spina bifida and control participants.
Quantification of Protein Concentrations in Cerebrospinal Fluid (CSF) 1 year The concentration of each identified protein in cerebrospinal fluid (CSF) will be measured using proteomic analysis. This outcome will report the amount of each protein present in the CSF, expressed in micrograms per milliliter (µg/mL) or nanograms per milliliter (ng/mL), allowing for comparison between patients with spina bifida and controls.
- Secondary Outcome Measures
Name Time Method Measurement of Neurogenesis in Median Ganglionic Eminence (MGE) Cultures 1 year The rate of neurogenesis in the median ganglionic eminence (MGE) of embryonic brain slice cultures will be measured following exposure to cerebrospinal fluid (CSF) from patients with Chiari II malformation and control subjects. The outcome will focus on the number of proliferating neurons per unit area, measured in cells per square millimeter (cells/mm²).
Measurement of Neuronal Migration in Median Ganglionic Eminence (MGE) Cultures 1 year The distance of neuronal migration in the median ganglionic eminence (MGE) of embryonic brain slice cultures will be measured following exposure to cerebrospinal fluid (CSF) from patients with Chiari II malformation and control subjects. The outcome will report the migration distance of neurons, measured in micrometers (µm).