Vitamin A Value of Spirulina Carotenoids in Humans
试验速览
- 阶段
- 1 期
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- The enrichment of spirulina ß-carotene and its cleavage product vitamin A were tracked after taken the oral dose of intrinsically labeled spirulina.
研究概览
简要总结
The goal of this protocol is to determine the vitamin A value (equivalence) of spirulina. The investigation will use intrinsically deuterium labeled spirulina and an isotope vitamin A reference dose, 13C10- retinyl acetate (13C10 RAc), in males (n=20). Up to 45 blood samples (10 ml/sample) will be collected from each subject over a two-month period to evaluate the bioavailability and bioconversion of spirulina ß-carotene to vitamin A.
详细描述
By utilizing novel stable isotope dilution techniques recently developed in our laboratory, we will trace deuterium labeled ß-carotene in intrinsically labeled spirulina, which is harvested from a hydroponic system. In the mean time, we will track 13C10 labeled vitamin A in humans. In this way we will determine absorption of ß-carotene from spirulina matrix and the conversion of the spirulina ß-Carotene to retinol. This will enable us quantitatively determine the vitamin A equivalence of spirulina.
The specific aims of this study are:
- To determine labeled ß-C blood response kinetics following an acute dose of intrinsically labeled spirulina;
- To determine labeled retinol blood response from the intrinsically labeled spirulina;
- To determine 13C10 retinol blood response from the isotope reference material 13C10 retinyl acetate;
- To determine s spirulina-vitamin A equivalence.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 40 Years 至 70 Years（Adult, Older Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy volunteer
排除标准
- •Not has GI track problems
- •Any medical condition that will affect the intestinal absorption
研究组 & 干预措施
1
干预措施: spirulina ß-carotene (Dietary Supplement)
2
干预措施: spirulina ß-carotene (Dietary Supplement)
结局指标
主要结局
The enrichment of spirulina ß-carotene and its cleavage product vitamin A were tracked after taken the oral dose of intrinsically labeled spirulina.
时间窗: 51 days
次要结局
未报告次要终点