ASSESSMENT OF A MORPHOFUNCTIONAL APPROACH TO TEMPOROMANDIBULAR JOINT ANKYLOSIS AND A PROTEOMIC AND GENOMIC STUDY OF THE DISEASE

Aimof the study

Assessment of a Morphofunctional Approach to TemporomandibularJoint Ankylosis and a Proteomic and Genomic Study of the Disease.

Objectivesof the study

1.               Comparison of a morphofunctional approach withconventional interpositional arthroplasty    for themanagement of TMJ ankylosis.

2.               To identify possible geneticmarkersof TMJ ankylosis by miRNA and exome sequencing

3.               To identify protein expression and molecularsignaling pathways involved in TMJ ankylosis  through phospho-proteomicevaluationand compare with normal controls.

4.               To propose a staging systemforTMJ ankylosis correlating the clinical, functional and radiographic features.

OBJECTIVE 1: Comparisonof a morphofunctional approach with conventional****interpositionalarthroplasty for the management of TMJ ankylosis.

21 cases of conventionalinterpositional arthroplasty [repositorydata and prospective cases to be operated at Yenepoya (Deemed to be) Universityand GSR Institue] will becompared with a minimum of 21 cases operatedat the GSR Institute, Hyderabad, using the morphofunctionaltechnique i.e Osteotomy modification+ cartilage graft for reconstruction

Maximum IncisalOpening (MIO) will be measured pre and post- operatively and will be comparedbetween the groups.

Incidence of reankylosiswill be assessed and compared between the groups

OBJECTIVE 3: Genetic basis of TMJankylosis

5 ml of venous bloodwill be collected from 5 patients with TMJ ankylosis and 5 healthy controls asdescribed previously and will be subjected to miRNA and exome sequencing

OBJECTIVE 3: Phospho-proteomic analysis of theankylotic mass

Resected ankyloticmasses from 5 patients with TMJ ankylosis and resected normal(non  ankylosed) condyles from 5 controls will be collected andstored as described previously and subjected to phospho-proteomic evaluation.

OBJECTIVE4: To propose a staging system for TMJ ankylosis correlating the****clinical,functionaland radiographic features.

Photographic,radiographic and functional assessment will be carried out to propose a stagingsystem for TMJ ankylosis based on the severity and extent of the disease.