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CAR-T-19 Cells for Patients With CD19+ Relapse/Refractory B-ALL

Phase 1
Recruiting
Conditions
CD19+ Relapse/Refractory B-ALL
Interventions
Biological: CAR-T-19 Cells
Registration Number
NCT05270772
Lead Sponsor
Chinese PLA General Hospital
Brief Summary

This is a single-arm, open-label phase I study to determine the safety, tolerability, and recommended dose (RD) of CAR-T-19 cells for patients with CD19+ relapse/refractory B-ALL under the age of 25.

Detailed Description

B-cell acute lymphoblastic leukemia (B-ALL) accounting for 85% of all ALL cases, is a common hematological malignancy in children and adults. CD19 is a widely expressed antigen in both normal B cells and B cells-derived leukemia and lymphomas. This is a single-arm, open-label phase I study of CAR-T-19 (anti CD19 scFv chimeric antigen receptor T) cells for patients with CD19-positive relapse/refractory B-ALL under the age of 25.

Primary endpoint is to determine the safety, tolerability, and recommended dose (RD) of CAR-T-19. Secondary endpoints measure ORR, OS and others.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
9
Inclusion Criteria
  • Patients with relapse/refractory B-ALL.
  • Tumor cells from bone marrow or peripheral blood are measured CD19+ with 3 months before enrollment.
  • Karnofsky Score ≥ 70 or Lansky Score ≥ 50.
  • Patients who have a life expectancy of at least 12 weeks.
  • Understand and voluntarily sign informed consent and are willing to comply with laboratory tests and other research procedures.
Exclusion Criteria
  • Patients with extramedullary relapse (EMR).
  • Patients with genetic diseases, Burkitt lymphoma and leukemia or other malignancies.
  • Patients positive for any of the following: HbsAg, HBV-DNA, HCV-Ab, HCV-RNA, HIV-Ab, TP-Ab, EBV-DNA or CMV-DNA.
  • Patients with other uncontrolled infection.
  • Patients who received anti-CD19 / anti-CD3 therapy, or any other anti-CD19 therapy.
  • Patients with active Grade II-IV GVHD within 3 months prior to screening.
  • Tumor cells are detected in cerebrospinal fluid.
  • Patients who received HSCT within 3 months prior to screening.
  • Anticipated other clinical trials within 4 weeks before this trial
  • Pregnant or lactating women.
  • Any condition that would, in the investigator's judgment, make it unsuitable for the donors to be enrolled.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
CAR-T-19 CellsCAR-T-19 CellsThe patients with CD19+ relapse or refractory B-ALL will receive a single infusion of autologous CAR-T-19 cells, with the escalated dose ranging from 0.5×10\^6/kg to 5.0×10\^6/kg CAR+ cells.
Primary Outcome Measures
NameTimeMethod
Dose limiting toxicity (DLT)up to 2 years

DLT within 28 days (±3 days) after CAR-T-19 infusion.

Maximum tolerated dose (MTD)up to 2 years

The maximum dose of DLT occurred in ≤1/6 of the subjects within 28 days (±3 days) after CAR-T-19 infusion.

Adverse eventsup to 2 years

Percentage of subjects with adverse events.

Secondary Outcome Measures
NameTimeMethod
Overall survival (OS)up to 2 years

The time from CAR-T-19 infusion to death from any cause.

Overall recovery rate (ORR)up to 2 years

Percentage of subjects who achieved complete response (CR) and incomplete hematologic recovery (CRi) for the first time at Day 28 and Day 90 after CAR-T-19 infusion.

MRD-Negative Rateup to 2 years

Percentage of subjects with MRD-negative CR and incomplete blood and MRD-negative CRi for the first time at Day 28 and Day 90 after CAR-T-19 infusion.

Duration of Response (DOR)up to 2 years

The time from achieving CR and CRi for the first time to recurrence or death from any cause

Progression-free Survival (PFS)up to 2 years

The time from CAR-T-19 infusion to recurrence, progression or death from any cause, whichever occurs first.

Trial Locations

Locations (1)

Chinese PLA General Hospital

🇨🇳

Beijing, Beijing, China

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