Perioperative Time Course of MMP-9 and Its Inhibitor During Carotid Artery Stenting and Carotid Endarterectomy

Completed

• Conditions: Ischemia Reperfusion Injury; Carotid Stenosis
• Interventions: Procedure: Carotid artery stenting; Procedure: Carotid endarterectomy

• Registration Number: NCT03410576
• Lead Sponsor: University of Pecs

Brief Summary

The aim was the comparison of the perioperative time courses of matrix metalloproteinase-9 (MMP-9) and its inhibitor (TIMP-1) during elective carotid artery stenting (CAS). The investigators used a matched, historical carotid endarteriectomy group as controls. Blood samples at four time points: T1: preoperative; T2: 60 minutes after stent insertion; T3: first postoperative morning; and T4: third postoperative morning. Plasma was isolated from heparin anticoagulated blood samples by low speed centrifugation at 4 °C, and stored at -80 °C until analyzed in a single batch at the end of the study. Plasma concentrations of MMP-9 and TIMP-1 were expressed as ng/ml.

Detailed Description

Not available

Study Timeline

• Study Start: 2013-10-01
• Primary Completion: 2015-11-30
• Study Completion: 2015-11-30
• Status Verified: 2018-01
• Study First Submitted: 2018-01-06
• Study First Submitted QC: 2018-01-18
• Last Update Submitted: 2018-01-18
• Study First Posted: 2018-01-25
• Last Update Posted: 2018-01-25

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

elective carotid artery surgery

Exclusion Criteria

Malignant diseases, inflammatory and systemic autoimmune disorders, psychiatric disorders and previous debilitating stroke

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Carotid artery stenting	Carotid artery stenting	Patients undergo carotid artery stenting.
Carotid endarterectomy	Carotid endarterectomy	Patients receive elective carotid endarterectomy.

Primary Outcome Measures

Name	Time	Method
Plasma MMP-9 concentrations	4 days	Plasma is isolated from heparin-anticoagulated blood samples by low speed centrifugation at 4 °C. MMP-9 levels were measured with quantitative sandwich enzyme-linked immunosorbent assay.
Plasma TIMP-1 concentrations	4 days	Plasma is isolated from heparin-anticoagulated blood samples by low speed centrifugation at 4 °C. TIMP-1 levels were measured with quantitative sandwich enzyme-linked immunosorbent assay.

Secondary Outcome Measures

Name	Time	Method
MMP-9/TIMP-1 ratio	4 days	MMP-9/TIMP-1 is calculated from the measured plasma MMP-9 and TIMP-1 concentrations

Trial Locations

Locations (1)

Department of Anaesthesiology and Intensive Therapy, Medical School, University of Pécs; 🇭🇺 Pécs, Baranya, Hungary