A Sequential Phase I/II Dose Escalation and Dose Selection Safety Study of Regional Intra-thrombus Plasmin (Human) Infusion In Acute Lower Extremity Native Artery or Bypass Graft Occlusion

Grifols Therapeutics LLC1 site in 1 country83 target enrollmentMarch 2007

ConditionsArterial Occlusive Diseases

Overview

Phase: Phase 1
Intervention: Not specified
Conditions: Arterial Occlusive Diseases
Sponsor: Grifols Therapeutics LLC
Enrollment: 83
Locations: 1
Primary Endpoint: Thrombolysis
Status: Completed
Last Updated: 9 years ago

Overview Investigators Eligibility Criteria Outcomes Sites Similar Trials

Overview

Brief Summary

The purpose of this study is to evaluate the safety of increasing doses of intra-thrombus Plasmin (Human) in acute peripheral arterial occlusion (aPAO). The ability of these Plasmin doses to dissolve the clots will be estimated by arteriography.

Detailed Description

There is an unmet need for proven thrombolytic agent in acute peripheral arterial occlusion (aPAO). The current assortment of plasminogen activators are slow to dissolve clots in the leg, and may lead to bleeding complications. Plasmin is a direct thrombolytic that may act more quickly when infused directly into the clot and thus assist in restoring blood flow to the leg. There is a large reserve in blood alpha-2 antiplasmin in the blood to rapidly inactivate Plasmin outside of the clot. Plasmin has the potential for an improved bleeding risk profile in aPAO.

Registry

clinicaltrials.gov

Start Date

March 2007

End Date

April 2010

Last Updated

9 years ago

Study Type

Interventional

Study Design

Single Group

Sex

All

Investigators

Sponsor

Grifols Therapeutics LLC

Responsible Party

Sponsor

Eligibility Criteria

Inclusion Criteria

•Age ≥ 18 years.
•Women of childbearing potential must use adequate contraception for the duration of the study and must have a negative pregnancy test prior to study entry.
•Unilateral limb ischemia: SVS acute ischemia Category I or IIa.
•Onset of symptoms \</= 14 days.
•Thrombosed (non-embolic) infrainguinal graft (synthetic, autologous, or single outflow composite) or infrainguinal native artery. For native arteries, only occlusions of ≥ 10 cm in length are eligible.
•Diagnosis of occlusive thrombus in the graft or artery by arteriography after Informed Consent is obtained.
•Ability to traverse the thrombus with a guidewire.
•Signed informed consent prior to study entry.

Exclusion Criteria

•Clinical evidence of significant disease that may interfere with the patient successfully completing the trial.
•Women who are pregnant or lactating, or first 10 days post-partum.
•Previous hemorrhagic stroke at any time. Thrombotic or embolic stroke or cerebrovascular events (including transient ischemic attack (TIA)) within one year.
•Intracranial or spinal neuro-surgery, or severe intracranial trauma in the last 3 months. Major surgery, organ biopsy, or major trauma within the last 10 days. Lumbar puncture or non-compressible arterial puncture in the last 10 days. Intra-ocular surgery within the last 10 days.
•Current bleeding diathesis. Active gastrointestinal or organ bleeding. Minor bleeding such as normal menses, cystitis, or minor hemorrhoidal bleeding are not exclusions.
•Uncontrolled arterial hypertension, defined as a systolic blood pressure \> 180 mmHg or diastolic blood pressure \> 110 mmHg.
•Known intracranial neoplasm, aneurysm, or arterio-venous malformation.
•Platelet count \< 75 x 10e9/L.
•Occlusion of a graft within 6 months of placement.
•Medically unable to tolerate an open vascular procedure.

Outcomes

Primary Outcomes

Thrombolysis

Time Frame: Approximately 5 hours after start of treatment

Thrombolysis at the end of treatment compared to baseline by arteriography

Secondary Outcomes

Thrombolysis(Approximately 2 hours after start of treatment)
Avoidance of both open surgical procedures and additional thrombolysis with a plasminogen activator or mechanical device thrombectomy.(30 days)
Avoidance of amputation(30 days)
Physiologic reperfusion defined as improvement in ankle brachial index (ABI)(End of treatment, post intervention procedures, Day 1 to 2, Day 7, and Day 30)
Avoidance of open surgical procedures(30 days)
Avoidance of additional catheter-directed thrombolysis with a plasminogen activator or mechanical device thrombectomy(30 days)
Patency assessed by duplex ultrasound imaging(Day 7 and Day 30)