Assessment of Protein Modification in Chronic Kidney Disease - Selected Clinical and Biochemical Aspects
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 195
- 试验地点
- 1
- 主要终点
- Biochemical parameters assessed in all groups part 2 - selected parameters of oxidative stress
研究概览
简要总结
The studies included the effect of chronic kidney disease advancement on the accumulation of oxidative stress markers in plasma. In patients with end-stage renal disease, the effect of replacement therapy was also assessed. Therefore, the patient with chronic kidney disease was evaluated divided into three groups (chronic kidney disease at stage G3b-G4, peritoneal dialysis, hemodialysis). In addition, changes in the interrelationship between oxidative modifications, carbonyl and nitrogen stress, and the carbamylation resulting from the progression of kidney disease have been taken into account. This issue is related to the assessment of whether the protein modification types differentiate patients depending on the stage of chronic kidney disease and the method of renal replacement therapy. Protein modifications associated with oxidative stress are a part of the complications resulting from chronic kidney diseases, such as malnutrition, chronic inflammation, dyslipidemia, iron disorder, and calcium and phosphate disorders. Also, diseases of atherosclerosis aetiology are much higher frequency in patients with chronic kidney disease than in those with normal kidney function. Therefore, in the studies presented here, particular attention was paid to the effect of oxidative stress on chronic kidney disease complications in the aspect of cardiovascular damage. The specificity of atherosclerosis in patients with chronic kidney disease was evaluated by comparing groups of this type of patients with patients with ischemic heart diseases and normal renal function.
详细描述
Redox imbalance in the course of CKD results in the intensification of oxidative and carbonyl stress, which leads to the modification of many molecules, including proteins necessary for the proper functioning of the body. The assessment of the accumulation of modified proteins in the plasma is not only an indirect indicator of the severity of redox imbalance in the system, but also allows the analysis of the influence of oxidative stress and its derivatives (glycation, carbonyl stress and carbamylation) on the pathogenesis of CKD. In addition, compounds formed as a result of the action of ROS on proteins may affect the development of long-term consequences of CKD, such as chronic inflammation, dyslipidemia, renal osteodystrophy, iron metabolism disorders and malnutrition. On the other hand, complications in patients with CKD may influence the intensification of oxidative modifications of proteins.
The following goals were set in the study:
- Assessment of the impact of CKD advancement on the severity of protein modification as a result of oxidative stress.
- Comparison of the effect of renal replacement therapy on protein modifications.
- Assessment of the relationship between selected protein modifications in CKD and complications typical of CKD
- Comparison of selected protein modifications in patients with CKD and patients with at least one history of a cardiovascular event.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- 未提供
排除标准
- 未提供
结局指标
主要结局
Biochemical parameters assessed in all groups part 2 - selected parameters of oxidative stress
时间窗: 4 years
CML \[µg/mg protein\], CEL \[µg/mg protein\], MG \[µg/mg protein\], AGE \[µg/mg protein\], RAGE \[µg/mg protein\] 3-NT \[µmol/mg protein\], AOPP \[µmol/mg protein\], carbonyl protein groups \[nmol/mg protein\], carbamyl protein groups \[µg/mg protein\]
Demographic data
时间窗: 4 years
age \[years\], sex \[number of female and male \[n\]\] were recorded in all groups
Biochemical parameters assessed in all groups part 1
时间窗: 4 years
The number of laboratory parameters were determined in all groups: * blood count: HGB \[g/dl\], RBC \[10\*12/l\], HCT \[l/l\], WBC \[10\*9/l\], PLT \[10\*9/l\] * iron metabolism parameters: Fe, UIBC,TIBC \[µg/dl\], ferritin \[ng/ml\] * glucose \[mg/dl\] * parameters of lipid metabolism \[mg/dl\] T-C, LDL-C, HDL-C, TG * parameters of hepatic metabolism \[U/l\]: activity of alanine transaminase, aspartate transaminase, alkaline phosphatase * creatinine \[mg/dl\], uric acid \[mg/dl\], urea \[mg/dl\], * creatinine will be combined with sex \[female/male\] and age \[years\] to report eGFR \[ml/min/1,73m\*2\] calculated on the basis on MDRD formula * albumin \[g/dl\] and total protein \[g/dl\] * Na \[mmol/l\], K \[mmol/l\] * parameters of calcium and phosphate metabolism: total calcium \[mg/dl\], ionised calcium \[mg/dl\], phosphates \[mg/dl\], PTH \[pg/ml\], FGF-23 \[pg/ml\], klotho \[ng/ml\] * selected parameters of inflammation: concentration of highly sensitive C-reactive protein (hsCRP) \[mg/l\]
次要结局
未报告次要终点
研究者
Dorota Formanowicz
MD.Ph.D. Associate Professor
Poznan University of Medical Sciences