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Randomized, Phase II Clinical Trial of Sulforaphane in Bladder Cancer Chemoprevention

Phase 2
Withdrawn
Conditions
Bladder Cancer
Bladder Tumor
Urothelial Carcinoma
Interventions
Other: Placebo Administration
Procedure: Standard of Care Surgery
Registration Number
NCT03517995
Lead Sponsor
H. Lee Moffitt Cancer Center and Research Institute
Brief Summary

The main purpose of this study is to see if Prostaphane is effective and can help reduce the progression of bladder cancer. Researchers also want to find out if Prostaphane is safe and tolerable, and to evaluate how Prostaphane works to reduce the progression of bladder cancer. This study will compare Prostaphane with a placebo to see if taking Prostaphane is better than taking a placebo. A placebo is a pill that looks like Prostaphane but has no drug or other active ingredients in it.

The study will be presented to eligible patients by the patient's surgeon at the time when an appointment is made for cystoscopy for suspicion of bladder cancer (BC) or to confirm BC diagnosis.

Detailed Description

The study will be presented to eligible patients by the patient's surgeon at the time when an appointment is made for cystoscopy for suspicion of bladder cancer (BC) or to confirm BC diagnosis.

Participants will be asked to spend 21 to 30 days in this study. The study will be conducted during the time from when the participant is diagnosed with bladder cancer to when they undergo a surgical procedure for the treatment or removal of their bladder cancer. The surgical procedure is done as a part of their regular medical care. Participants will be asked to come for 1 additional visit as part of this research study at the midpoint between their biopsy and surgery.

Recruitment & Eligibility

Status
WITHDRAWN
Sex
All
Target Recruitment
Not specified
Inclusion Criteria
  • Men and women; age ≥18 years; evidence of non-muscle invasive or muscle invasive primary bladder tumor (urothelial carcinoma +/- variant histology) discovered on cystoscopy or radiologic imaging performed within 60 days of randomization; with no evidence of distant metastases; planned Transurethral Resection+B21 (TURBT), cystoscopy with biopsies or cystectomy (total or partial);
  • Absent prior pelvic radiation; normal organ function;
  • Absent neoadjuvant chemotherapy (refusal or ineligibility); (the participant may have prior intravesical treatment exposure (including Bacillus Calmette-Guerin (BCG), mitomycin, gemcitabine, valrubicin, docetaxel, etc.) for bladder cancer (BC) (excluding primary bladder radiation therapy) provided that treatment was completed greater than 30 days prior to the patient's randomization visit);
  • Non-smokers (urinary cotinine tested);
  • Agree to restrict dietary sources of Sulforaphane (SFN) to 3 or 5 servings/week and abstain from consuming SFN supplements beginning three days prior to start of study and throughout duration of the study;
  • Eastern Cooperative Oncology Group (ECOG) performance status 0-2;
  • Willing to discontinue current vitamin/mineral supplement use and substitute with a standard multivitamin supplement provided for the study;
  • Willing to use an effective method of contraception, if the partner is of child-bearing age, while on study;
  • Willing to comply with proposed visit and treatment schedule;
  • Able to understand and willing to sign a written informed consent document;
  • Participants must have normal organ and marrow function.
Exclusion Criteria
  • Evidence of other cancers (excluding non-melanoma skin cancer) or metastatic disease;
  • Prior pelvic radiation; concurrent systemic chemotherapy for any other cancer, excluding non-melanoma skin cancer;
  • Any treatment for the bladder tumor other than intravesical therapy;
  • Prior treatment with a known histone deacetylase inhibitor (including but not limited to valproic acid, suberoylanilide hydroxamic acid (SAHA), Panobinostat (LBH589), etc.) within 6 months prior to starting study treatment or while on study therapy;
  • Current treatment with warfarin;
  • Use of dietary supplements or herbal remedies which may affect the study outcome - unless the participant is willing to discontinue taking them for 1 month prior to starting study;
  • Usual consumption of > 5 servings per week of brassica vegetables;
  • Gastrointestinal ailments which would interfere with the ability to adequately absorb SFN;
  • Allergy/known intolerance to cruciferous vegetables;
  • Used antibiotics (more than 3 doses) within 10 days prior to study (day -14 prior to study randomization);
  • Current smoker.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Sulforaphane Plus SurgeryStandard of Care SurgerySulforaphane Administration prior to bladder cancer surgery.
Placebo Plus SurgeryPlacebo AdministrationPlacebo Administration prior to bladder cancer surgery.
Placebo Plus SurgeryStandard of Care SurgeryPlacebo Administration prior to bladder cancer surgery.
Sulforaphane Plus SurgerySulforaphane AdministrationSulforaphane Administration prior to bladder cancer surgery.
Primary Outcome Measures
NameTimeMethod
Magnitude of ChangeUp to 30 days

Magnitude of change in Intermediate Endpoint Biomarkers (IEBs) of proliferation (Ki-67 expressing cells- an independent marker of poor prognosis in bladder cancer (BC)) from baseline to end of treatment with 20 mgs Prostaphane® \[Nutinov Labs, France\] containing 200 μmol of Sulforaphane (SFN) a day at 3-4 weeks (maximum 30 days) in BC cells and benign/adjacent cells.

Secondary Outcome Measures
NameTimeMethod
Occurrence of Adverse Events per Study ArmEnd of study, approximately 30 days

Safety of SFN at this dose (vs. Placebo) as indicated by incidence of adverse events and toxicities, monitored using Common Toxicity Criteria version 5.0, complete blood count (CBC), and complete metabolic panel (CMP) from baseline at mid-point and at end of trial.

Effectiveness of Sulforaphane vs. PlaceboEnd of study, approximately 30 days

Effectiveness of SFN at this dose (vs. placebo) as indicated by modulation of other IEBs of proliferation, apoptosis and phase II enzymes, as well as the potential molecular mechanism of SFN, we will measure changes in: (i) BC histology grade; (ii) labeling index of a sensitive biomarker that is a member of DNA replication origin licensing complex, Mcm2; (iii) apoptosis (Caspase-3); (iv) Phase II enzymes (glutathione transferases, epoxide hydrolase, Nicotinamide adenine dinucleotide phosphate (NAD(P)H): quinone reductase, and glucuronosyltransferases); (v) Nrf2 and Transcription factor (NF-kB) signaling, from baseline to end of treatment in BC cells and benign/adjacent cells.

Mid-study Bioavailability of SulforaphaneMid-study, approximately 15 days

Bioavailability, of SFN at this dose vs. Placebo. Investigators will measure change in SFN in plasma and bladder tissue from baseline, at mid-point and at end of study.

End of Study Bioavailability of SulforaphaneEnd of study, approximately 30 days

Bioavailability, of SFN at this dose vs. Placebo. Investigators will measure change in SFN in plasma and bladder tissue from baseline, at mid-point and at end of study.

Adherence of Sulforaphane vs. PlaceboEnd of study, approximately 30 days

Adherence based on pill counts and diet and pill logs from baseline.

Acceptability of Sulforaphane vs. PlaceboEnd of study, approximately 30 days

Acceptability based on pill counts and diet and pill logs from baseline.

Trial Locations

Locations (2)

H. Lee Moffitt Cancer Center and Research Institute

🇺🇸

Tampa, Florida, United States

James A. Haley Veteran's Administration Hospital

🇺🇸

Tampa, Florida, United States

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