Effect of Subtle Energy Transmission and Tao Calligraphy Mindfulness Practice on Mitochondrial DNA (mtDNA) in Peripheral Blood Leukocytes
试验速览
- 阶段
- 不适用
- 状态
- Enrolling By Invitation
- 发起方
- 入组人数
- 50
- 试验地点
- 2
- 主要终点
- Mean Change from Baseline in Mitochondrial-to-nuclear genome ratio (Mitochondrial DNA content) in peripheral blood Leukocytes at 3 months, 6 months and 12 months
研究概览
简要总结
The goal of this pilot clinical trial is to learn if a subtle energy transmission and Tao Calligraphy Mindfulness Practice works to improve mitochondrial DNA. The main questions it aims to answer are:
- Does Tao Calligraphy Mindfulness practice improve mitochondrial DNA content in peripheral blood leucocytes in adults?
- Will this improvement of mitochondrial DNA content in peripheral blood leucocytes be statistically significant? Researchers will compare the values of mitochondrial DNA content in peripheral blood leucocytes at beginning of the mindfulness practices to the values at 3 months, 6 months and 12 months of practice group.
Participants will:
- have blood samples will drawn at accredited institutions and analyzed for Mitochondrial DNA content in Leucocytes upon entry into the study - Baseline time point; at 3-months time point, at six-months time point and at twelve-months time point.
- All participants will complete the set of 3 questionnaires upon entry into the study - Baseline time point; at 3-months time point, at six-months time point and at twelve-months time point.
- Practice the Mindfulness with Tao Calligraphy minimum 30 minutes daily.
详细描述
Objective
The goal of this study is to measure the effects of a unique form of Tao Calligraphy mindfulness practice that involves engaging with Tao art (tracing the Tao Calligraphy "Greatest Love" while listening to or singing the Tao Music or Tao Song "Greatest Love") together with subtle energy transmission on Mitochondrial DNA in peripheral blood leucocytes.
In our previous study, the participants who regularly practiced, shown an increase of the length in telomeres in peripheral blood granulocytes. In our other studies, participants reported a decrease in symptoms of their illness as perceived subjectively, improved signs reported by treating clinicians and an improvement of well-being as measured by standardized scientific questionnaires.
Hypotheses
The research null hypothesis is that individuals who receive subtle energy transmission for mitochondrial DNA in hemato-lymphoid tissue, and who will engage in daily Tao Calligraphy mindfulness practice for 12 months, will have no significant change in Mitochondrial DNA content in peripheral blood leucocytes in follow-up analyses and will show no improvement of well-being as measured by standardized scientific questionnaire Rand SF-36 at 3, 6 and 12 months.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Other
- 盲法
- None
入排标准
- 年龄范围
- 19 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 19 and over
- •Willingness and ability to comply with data collection requirements
- •Submission of required documentation before entering the study, including informed consent and consent to release of information
- •Healthy or Ill, with the exception of genetic illnesses and cancer (for which treatments could negatively impact measurement) and serious mental disorders (e.g. bipolar disorder, schizophrenia, psychosis),
- •Willingness to allow their data to be used for research purposes and published as deemed fit (while conforming to all applicable privacy laws) by Sha Research Foundation.
- •Willingness to practice the daily calligraphy meditations and follow the protocol.
排除标准
- •Not meeting any of the inclusion criteria
- •Bipolar disorders, other serious mental disorders (e.g. schizophrenia, psychosis), genetic illnesses (primarily affected chromosomes), and cancer (treatment could negatively impact telomere during research period)
- •inability to sign consent and follow instructions
- •Unwillingness to participate in data gathering
- •Unable to follow the practice regimen, including the daily calligraphy meditations
- •Pregnant or nursing. Participants who become pregnant during the study will be required to end their participation. (to avoid any, at current time unknown, potential negative effect of the study on the fetus).
- •There are no exclusion criteria placed upon potential subjects related to national origin, culture, ethnicity, race, sex, physical disability, sexual orientation, religion, or spiritual practices.
研究组 & 干预措施
The Practice Group
The Practice group will start practices at baseline time-point and will stop practices after 12 months at 12-months time-point. The participants in Practice group will practice Mindfulness practice with Calligraphy at least 30 minutes daily for 12 months. All participants in Practice group will have the blood drawn for Mitochondrial DNA content analysis and complete the SF-36 questionnaire upon entry into the study - Baseline time-point; at 3-months time-point, at six-months time point and at twelve-months time point.
干预措施: Mindfulness practice with Tao Art (Calligraphy and Song Greatest Love) (Other)
结局指标
主要结局
Mean Change from Baseline in Mitochondrial-to-nuclear genome ratio (Mitochondrial DNA content) in peripheral blood Leukocytes at 3 months, 6 months and 12 months
时间窗: From start of Mindfulness practices with Tao Art (Calligraphy and Song Greatest Love) to end of practices at 12 months
Genomic DNA is extracted from peripheral blood and DNA concentration and purity are assessed by spectrophotometer. Mitochondrial DNA content in leukocytes is quantified by real-time quantitative PCR. Calculating Mitochondrial DNA content is done as follows: Ct values for Mitochondrial DNA targets are normalized to the nuclear gene Ct using the delta-delta cycle threshold method, using equation: Mitochondrial DNA content = relative Mitochondrial DNA abundance / nuclear DNA abundance. This ratio reflects the relative number of mitochondrial genomes per nuclear genome in leukocytes. The normal range for the mitochondrial-to-nuclear genome ratio (Mitochondrial DNA/ Nuclear DNA) in human blood leukocytes is approximately 1.2 to 1.8.
Mean Change from Baseline in Mitochondrial-to-nuclear genome ratio (Mitochondrial DNA content) in peripheral blood Leukocytes at 3 months, 6 months and 12 months
时间窗: From start of Mindfulness practices with Tao Art (Calligraphy and Song Greatest Love) to end of practices at 12 months
Genomic DNA is extracted from peripheral blood and DNA concentration and purity are assessed by spectrophotometer. Mitochondrial DNA content in leukocytes is quantified by real-time quantitative PCR. Calculating Mitochondrial DNA content is done as follows: Ct values for Mitochondrial DNA targets are normalized to the nuclear gene Ct using the delta-delta cycle threshold method, using equation: Mitochondrial DNA content = relative Mitochondrial DNA abundance / nuclear DNA abundance. This ratio reflects the relative number of mitochondrial genomes per nuclear genome in leukocytes. The normal range for the mitochondrial-to-nuclear genome ratio (Mitochondrial DNA/ Nuclear DNA) in human blood leukocytes is approximately 1.2 to 1.8.
次要结局
- Mean Change from Baseline in Quality of Life Scores on 36-item short-form Quality of Life Questionnaire (SF-36) at 3 months, 6 months and 12 months(From start of Mindfulness practices with Tao Art (Calligraphy and Song Greatest Love) to end of practices at 12 months.)
- Mean Change from Baseline in Quality of Life Scores on 36-item short-form Quality of Life Questionnaire (SF-36) at 3 months, 6 months and 12 months(From start of Mindfulness practices with Tao Art (Calligraphy and Song Greatest Love) to end of practices at 12 months.)