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Repeat Infusion of Autologous Bone Marrow Cells in Multiple Sclerosis

Not Applicable
Completed
Conditions
Multiple Sclerosis
Interventions
Procedure: Infusion of autologous bone marrow
Registration Number
NCT01932593
Lead Sponsor
North Bristol NHS Trust
Brief Summary

There is no cure for Multiple Sclerosis (MS) and we are always looking at new ways to stop the disease process and/or promote repair.

We hypothesise that autologous bone marrow cellular therapy in chronic MS offers durable benefit.

The purpose of this study is to test the safety of repeated bone marrow stem cell infusion in patients with MS. We want to find out what effects, good and/or bad, it has on you and your disability.

You have previously participated in a safety study of bone marrow stem cell infusion in patients with MS. The results raised the possibility of some early partial repair; measurements of the speed of neurological impulses in the brain and spinal cord improved. The current study seeks to determine whether those benefits have persisted and whether they can be repeated or enhanced by repeating the procedure.

Detailed Description

On the background of our own and others' experimental BM stem cell studies, we recently completed a phase 1 feasibility/safety trial of BM cell therapy in 6 patients with longstanding progressive MS (www.nature.com/clpt/journal/v87/n6/full/clpt201044a.html). Safety was confirmed, and intensive serial neurophysiological tests showed statistically significant improvements at 12 months. While highly preliminary and entirely uncontrolled, these results at least raise the possibility of a beneficial effect within the damaged central nervous system (CNS). A phase 2 clinical trial to formally assess efficacy of intravenous infusion of autologous bone marrow cells in progressive MS will commence in the near future (ACTiMuS trial). This trial comprises a programme of translational and clinical stem cell research, aiming (1) to continue translation with a phase two controlled trial of autologous bone marrow cells (BMCs) in chronic MS; and (2) to explore in parallel the potential mechanisms of action by studying BM cells from treated patients and controls, aiming to establish which BM sub-population(s) contribute(s) to efficacy, and which reparative mechanism(s) are important.

It is not known whether repeated infusion of autologous bone marrow offers additional benefit or how long improvements might be expected to last. The current proposal seeks to explore whether the neurophysiological improvements observed in the phase I study persist several years after the initial single infusion and whether these can be either replicated or augmented by an additional infusion of autologous bone marrow cells.

Hypothesis and aims

We hypothesise that intravenously-delivered autologous bone marrow cellular therapy (BMCT) in chronic MS offers significant benefit. We hypothesize also that the mechanisms are multiple, and include immunomodulation and reparative and/or neuroprotective effects within the CNS; and are offered by one or more BM stem cell sub-populations, jointly contributing to the therapeutic impact. Exploring and understanding these mechanisms, and the biology of the cells responsible, will allow the development of more effective reparative cell therapy in MS.

The current study seeks to examine whether the observed improvements noted in conduction times in central nervous system pathways in the phase I 'Study of Intravenous Autologous Marrow in Multiple Sclerosis (SIAMMS)' persist several years following the initial single infusion and whether these can be either replicated or augmented by an additional infusion of autologous bone marrow cells and analysis of research samples will be performed as per samples included in the concurrent phase 2 clinical trial 'Assessment of bone marrow-derived cellular therapy in progressive multiple sclerosis (ACTiMuS)' (REC 12/SW/0358, ISRCTN27232902, NCT01815632).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
4
Inclusion Criteria

Participation in the phase I safety and feasibility 'Study of Intravenous Autologous Marrow in Multiple Sclerosis' (SIAMMS) (REC reference number number 05/Q1704/137 Clin Pharmacol Ther. 2010 Jun;87(6):679-85)

Exclusion Criteria
  • pregnancy, breastfeeding or lactation
  • bone marrow insufficiency
  • history of lymphoproliferative disease or previous total lymphoid irradiation immune deficiency
  • history of current or recent (<5 years) malignancy
  • chronic or frequent drug-resistant bacterial infections or presence of active - infection requiring antimicrobial treatment
  • frequent and/or serious viral infection
  • systemic or invasive fungal disease within 2 years of entry to study
  • significant renal, hepatic, cardiac or respiratory dysfunction
  • contraindication to anaesthesia
  • bleeding or clotting diathesis
  • current or recent (within preceding 12 months) immunomodulatory therapy other than corticosteroid therapy
  • treatment with corticosteroids within the preceding 3 months
  • radiation exposure in the past year other than chest / dental x-rays
  • previous claustrophobia
  • the presence of any implanted metal or other contraindication to MRI participation in another experimental study or treatment within previous 24 months

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Infusion of autologous bone marrowInfusion of autologous bone marrowBone marrow harvest under general anaesthetic and intravenous infusion of filtered but otherwise unselected autologous bone marrow
PlaceboInfusion of autologous bone marrow-
Primary Outcome Measures
NameTimeMethod
Adverse events1 year post-infusion of autologous bone marrow

Number of adverse events

Secondary Outcome Measures
NameTimeMethod
Global evoked potentialBaseline then 6 months and 12 months post-infusion of autologous bone marrow

The 'global evoked potential (GEP)' has been developed as a tool that, by combining multimodal evoked potential recordings to a single score, may be used to monitor the evolution of MS in individual patients, and as a surrogate end point in clinical trials.

MRI brainBaseline and 6 months post-infusion of autologous bone marrow

MRI brain scan

Expanded disability status scale (EDSS)Baseline then 6 months and 12 months post-infusion of autologous bone marrow

Expanded disability status scale - clinical scale of disability used in MS trials

Multiple sclerosis functional composite (MSFC)Baseline then 6 months and 12 months post-infusion of autologous bone marrow

The MSFC is a three-part quantitative assessment of disability and includes a timed walk, the nine-hole peg test and the Paced Auditory Serial Addition Test (PASAT).

Multiple sclerosis impact scale (MSIS-29)Baseline then 6 months and 12 months post-infusion of autologous bone marrow

Self-reporting questionnaire re impact of MS

Trial Locations

Locations (1)

North Bristol NHS Trust

🇬🇧

Bristol, Avon, United Kingdom

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