Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise

Not Applicable

Completed

• Conditions: Metabolism in Adipose Tissue
• Interventions: Other: Repeated 30-s sprint exercise

• Registration Number: NCT04566354
• Lead Sponsor: Karolinska Institutet

Brief Summary

Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.

Detailed Description

PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.

METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.

SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports

Basic Information
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Recruitment & Eligibility
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Study & Design
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Trial Locations

Study Timeline

• Study Start: 2015-09
• Study First Submitted: 2015-09-22
• Primary Completion: 2016-09
• Study Completion: 2017-09
• Status Verified: 2020-09
• Study First Submitted QC: 2020-09-22
• Last Update Submitted: 2020-09-22
• Study First Posted: 2020-09-28
• Last Update Posted: 2020-09-28

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 18

Inclusion Criteria

• Physical active
• Healthy

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Exclusion Criteria

• Smokers
• Pregnant
• On medication
• No infection
• No asthma

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Sprint exercise	Repeated 30-s sprint exercise	Three bouts of 30-s sprint exercise with 20 min rest in between. Three fat biopsies obtained ar rest before first sprint, 15 min after and 120 min after third sprint Blood samples from stomach vein and arteria during the whole experiment

Primary Outcome Measures

Name	Time	Method
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise	After 30-s repeated sprint exercise	Stomach fat biopsies and arterial and stomach vein blood are obtained

Secondary Outcome Measures

Name	Time	Method
Changes in blood flow in adipose tissue	After 30-s repeated sprint exercise	The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work.
Changes in different metabolites	After 30-s repeated sprint exercise	Analysed for lactate, ammonia and IL-6

Trial Locations

Locations (1)

Karolinska Institutet/Karolinska University Hospital; 🇸🇪 Stockholm, Sweden