The Effect of Long-term Exercise on the Production of Osteoclastogenic and Anti-osteoclastogenic Cytokines by Peripheral Blood Mononuclear Cells and on Serum Markers of Bone Metabolism
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 43
- 试验地点
- 1
- 主要终点
- Evidence of altered bone resorption and/or production as measured by measurements of serum levels of osteocalcin and degradation products of Type I collagen (C-terminal telopeptides)
研究概览
简要总结
This is the second phase of a study designed to determine the immunological effects of long-term exercise on risk factors for ischemic heart disease (phase 1) and osteoporosis (phase 2). The results indicate that six months of moderate intensity exercise reduces bone resorption and increases the secretion of anti-osteoclastogenic cytokines by peripheral blood mononuclear cells.
详细描述
In a before and after trial involving 43 healthy adults the investigators measured the effect of six months of supervised exercise on the spontaneous and phytohemagglutinin-induced production of osteoclastogenic cytokines (interleukin-1α, tumor necrosis factor-α), anti-osteoclastogenic cytokines (transforming growth factor-β, interleukins 4 and10), pleiotropic cytokines with variable effects on osteoclastogenesis (interferon-γ, interleukin-6), and T cell growth and differentiation factors (interleukins 2 and 12) by peripheral blood mononuclear cells. The investigators also measured lymphocyte phenotypes, and serum markers of bone formation (osteocalcin), bone resorption (C-terminal telopeptides of Type I collagen), and bone homeostasis (estradiol, vitamin D2, testosterone, parathyroid hormone, insulin-like growth factor).
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 30 Years 至 60 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Normal exercise treadmill test
排除标准
- •Any medical condition that would prevent participation in a six month exercise program
结局指标
主要结局
Evidence of altered bone resorption and/or production as measured by measurements of serum levels of osteocalcin and degradation products of Type I collagen (C-terminal telopeptides)
时间窗: 6 months
Evidence of altered secretion of osteoclastogenic cytokines by peripheral blood mononuclear cells
时间窗: 6 months
secretion of IL-1 alpha, TNF-alpha, IFN-gamma by cultured peripheral blood mononuclear cells
Evidence of altered secretion of anti-osteoclastogenic cytokines by cultured peripheral blood mononuclear cells
时间窗: 6 months
secretion of IL-10, IL-4, TGF-beta, IL-6 by cultured peripheral blood mononuclear cells
Evidence of altered blood mononuclear cell phenotypes
时间窗: 6 months
Immunophenotyping of blood mononuclear cells by FACScan flow cytometer
Evidence of altered serum factors of bone homeostasis
时间窗: 6 months
Measurements of serum levels of estradiol, testosterone, PTH, IGF-1
Evidence of altered secretion of growth and differentiation factors by cultures peripheral blood mononuclear cells
时间窗: 6 months
Measurements of IL-2, IL-4, IL-12
次要结局
未报告次要终点
研究者
John Kelly Smith
Emeritus Professor of Medicine
East Tennessee State University