The Influence of Different Concentrations of Glucose in Culture Media on In Vitro Fertilization (IVF) Outcome of Sibling Oocytes

• Conditions: Infertility
• Interventions: Other: Sibling oocyte incubation

• Registration Number: NCT02066584
• Lead Sponsor: Hillel Yaffe Medical Center

Brief Summary

The importance of glucose in culture media on fertilization and embryo development is still controversial. Although it has been reported that glucose is essential for fertilization process and biosynthesis of membrane lipid and nucleic acids, comparatively high levels of glucose in culture media may cause developmental arrest of cleavage stage embryos. The aim of this retrospective study is to examine whether exposure of sibling oocytes to different concentrations of glucose in culture media has any further effect on IVF outcome.

Detailed Description

This retrospective study will include data from either standard IVF or Intracytoplasmic Sperm Injection (ICSI) cycles, in which sibling oocytes were equally divided and incubated separately in one of the following pairs of culture media:

1. Universal IVF medium vs. ISM1 medium ( Origio, Medi-Cult)

2. P1 medium vs. ECM medium (Irvine).

Fertilization rate,as well as embryo cleavage rate and morphology will be evaluated.

Study Timeline

• Status Verified: 2014-02
• Study First Submitted: 2014-02-16
• Study First Submitted QC: 2014-02-18
• Last Update Submitted: 2014-02-18
• Study First Posted: 2014-02-19
• Last Update Posted: 2014-02-19
• Study Start: 2014-04
• Primary Completion: 2016-04
• Study Completion: 2016-04

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: Female
• Target Recruitment: 600

Inclusion Criteria

• Sibling oocytes from cycles where at least 4 oocytes were retrieved

Exclusion Criteria

• Less than 4 oocytes retrieved
• Partner has undergone testicular sperm extraction (TESE)

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Universal IVF Medium	Sibling oocyte incubation	An oocyte culture medium containing 5.55 Mm glucose (Origio, Medi-Cult)
ECM Medium	Sibling oocyte incubation	An oocyte culture medium containing 0.5mM glucose (Irvine)
P1 Medium	Sibling oocyte incubation	An oocyte culture medium containing no glucose (Irvine)
ISM1 Medium	Sibling oocyte incubation	An oocyte culture medium containing 1mM glucose ((Origio, MediCult)

Primary Outcome Measures

Name	Time	Method
Fertilization Rate	2 years	Fertilization rates will be retrospectively compared according to culture media in which sibling oocytes were incubated

Secondary Outcome Measures

Name	Time	Method
Embryo Quality	2 years	Embryo quality and developmental rate will be retrospectively compared according to culture media in which sibling oocytes were incubated

Trial Locations

Locations (1)

Hillel Yaffe Medical Center; 🇮🇱 Hadera, Israel